Sulfate reduction by a haloalkaliphilic bench-scale sulfate-reducing bioreactor and its bacterial communities at different depths
Sulfate reduction by a haloalkaliphilic bench-scale sulfate-reducing bioreactor and its bacterial communities at different depths
复制标题
卤碱小型硫酸盐还原生物反应器还原硫酸盐及其不同深度的细菌群落
DOI：
10.1016/j.bej.2019.04.008
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发表时间：
2019-07-15
影响因子：
3.9
通讯作者：
Yang, Maohua
中科院分区：
文献类型：
--
作者：
Mu, Tingzhen;Xing, Jianmin;Yang, Maohua
从高钠浓度和高pH的废水中有效去除高浓度硫酸盐是迄今为止的挑战。本研究采用小型嗜盐碱生物反应器连续运行，高效处理模拟废水中高浓度硫酸盐。结果表明，当进水硫酸盐浓度为3000 mgl（-1），Na+浓度为1.0 M，pH值为9.5时，在水力停留时间为24 h内，硫酸盐的去除率可达70%。硫酸盐的去除率达到2.12 gl（-1）d（-1），远高于文献结果。反应器底部区域对总硫酸盐还原的贡献约为40%。随着深度的减小，其速率逐渐减小。有机酸的组成与反应器深度密切相关。微生物群落分析表明，脱硫弧菌对硫酸盐的去除起着最重要的作用，其相对丰度在底部区域最高。随着反应器深度的减小，丰度逐渐降低。此外，这些结果还揭示了硫酸盐去除的动态过程和整个生物反应器中细菌群落的多样性。通过16 S rRNA序列分析鉴定了多种微生物，加深了对硫酸盐还原菌与非硫酸盐还原菌在硫酸盐去除过程中相互作用的认识。该工艺的高效运行为工业废水的生物除硫提供了巨大的潜力。
Efficient removal of high concentration sulfate from wastewater with high sodium concentration and pH is a challenge to date. In this study, a bench-scale haloalkaliphilic bioreactor was continuously operated for high-efficiency treatment of high concentration sulfate in simulation wastewater. It was found that up to 70% of sulfate was removed within hydranlic retention time of 24 h when sulfate concentration in influent was as high as 3000 mgl(-1) at 1.0 M Na+ and pH 9.5. Sulfate removal rate reached 2.12 gl(-1) d(-1), which was much higher than results in literatures. The bottom region of bioreactor contributed approximate sulfate removal of 40% to total sulfate reduction. Then, the rate decreased gradually with the reduction of depth. Moreover, the composition of organic acids was closely related to depth of the bioreactor. According to microbial community analysis, Desulfonatronovibrio might play the most important role in sulfate removal, and its relative abundance was the highest at bottom region. The abundance decreased gradually as reduction of the depth of bioreactor. In addition, these results revealed a dynamic process of sulfate removal and variety of bacterial communities in whole bioreactor. The identification of many microorganisms by 16S rRNA sequencing deepened the insight of SRB interaction with non-SRB in sulfate-removal process. The high-efficiency process offered a great biotechnological penitential for sulfate removal of industrial wastewater.