A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain.
A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain.
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DOI：
10.1038/ncomms12138
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发表时间：
2016-07-08
影响因子：
16.6
通讯作者：
Ma JF
中科院分区：
文献类型：
--
作者：
Huang XY;Deng F;Yamaji N;Pinson SR;Fujii-Kashino M;Danku J;Douglas A;Guerinot ML;Salt DE;Ma JF
大米是世界上一半以上人口卡路里和矿物质营养物质的主要来源。然而，人们对水稻中铜（Cu）积累变异的遗传基础知之甚少，铜是一种必需但有潜在毒性的营养物质。在这里，我们确定OsHMA4可能是水稻籽粒中控制铜积累的数量性状位点的致病基因。我们提供的证据表明，OsHMA4的功能是将Cu隔离到根液泡中，限制Cu在籽粒中的积累。籽粒铜积累的差异很可能是由于单个氨基酸取代导致不同的OsHMA4运输活性。在调查的1367份水稻材料中，有67份存在与低粒铜相关的等位基因。鉴定OsHMA4基因的天然等位基因变异可能有助于培育籽粒铜浓度与土壤中铜浓度和日粮需求相适应的水稻品种。铜（Cu）是一种必需的矿质营养素，但在稻米中浓度过高会引起毒性。在这里，作者提供的证据表明，水稻籽粒铜浓度的自然变化是由于OsHMA4转运体中的单个氨基酸取代导致铜在根液泡中的固存改变引起的。
Rice is a major source of calories and mineral nutrients for over half the world's human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs. Copper (Cu) is an essential mineral nutrient but high concentrations in rice grain can cause toxicity. Here the authors provide evidence that natural variation in rice grain Cu concentration is caused by altered sequestration of Cu into root vacuoles due to a single amino acid substitution in the OsHMA4 transporter.