CHROMOSOME-SPECIFIC RECOMBINANT-DNA LIBRARIES FROM THE FUNGUS ASPERGILLUS-NIDULANS
CHROMOSOME-SPECIFIC RECOMBINANT-DNA LIBRARIES FROM THE FUNGUS ASPERGILLUS-NIDULANS
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DOI：
10.1093/nar/19.11.3105
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发表时间：
1991-06-11
影响因子：
14.9
通讯作者：
TIMBERLAKE, WE
中科院分区：
文献类型：
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作者：
BRODY, H;GRIFFITH, J;TIMBERLAKE, WE
通过鉴定含有长染色体DNA插入片段的重叠重组DNA克隆，促进了物理基因组图谱的开发。 为了简化确定基因组文库中哪些克隆彼此重叠所需的分析，我们将构巢曲霉粘粒文库划分为染色体特异性亚组。 八个A。通过脉冲场凝胶电泳解析巢蛋白染色体，并与粘粒文库的过滤复制品杂交。 获得的子集合似乎是基于子集合大小和染色体长度之间的对应关系的染色体的代表。 在每个染色体特异性子集合中获得足够数量的克隆，以预测单个克隆的重叠和组装成有限数量的连续区域。 这种方法应该适用于许多生物体的基因组可以解决脉冲场凝胶电泳。
Development of physical genomic maps is facilitated by identification of overlapping recombinant DNA clones containing long chromosomal DNA inserts. To simplify the analysis required to determine which clones in a genomic library overlap one another, we partitioned Aspergillus nidulans cosmid libraries into chromosome-specific subcollections. The eight A. nidulans chromosomes were resolved by pulsed field gel electrophoresis and hybridized to filter replicas of cosmid libraries. The subcollections obtained appeared to be representative of the chromosomes based on the correspondence between subcollection size and chromosome length. A sufficient number of clones was obtained in each chromosome-specific subcollection to predict the overlap and assembly of individual clones into a limited number of contiguous regions. This approach should be applicable to many organisms whose genomes can be resolved by pulsed field gel electrophoresis.