Transcriptional responses of Acropora hyacinthus embryo under the benzo(a)pyrene stress by deep sequencing
Transcriptional responses of Acropora hyacinthus embryo under the benzo(a)pyrene stress by deep sequencing
复制标题
通过深度测序研究苯并芘胁迫下卫城风信子胚胎的转录反应
DOI：
10.1016/j.chemosphere.2018.04.149
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发表时间：
2018-09-01
期刊：
影响因子：
8.8
通讯作者：
Diao, Xiaoping
中科院分区：
文献类型：
--
作者：
Xiao, Rong;Zhou, Hailong;Diao, Xiaoping
珊瑚胚胎是珊瑚早期生长发育的关键和敏感时期。苯并（a）芘（BaP）广泛分布于海洋中，具有很强的毒性，但对暴露于这种广泛存在的环境污染物的珊瑚胚胎的毒性影响的资料很少。因此，在本研究中，我们利用Illumina Hiseg（TM）4000平台来探索鹿角珊瑚胚胎在BaP胁迫下的基因响应。共获得并分析了130，042个Unigenes，其中约37.67%与来自四个不同物种的序列相匹配。总共有2606个Unigenes被上调，3872个Unigenes被下调。经过GO（Gene Ontology）注释，结果表明，“细胞过程”和“代谢过程”是生物学过程的主导范畴，其中“结合”和“催化活性”是分子功能中最丰富的子范畴。根据京都基因和基因组百科全书（KEGG）分析，富集了差异表达最多的基因，以及在氧化磷酸化途径、外源物质代谢、免疫相关基因、凋亡和人类疾病基因中表达下调的基因。同时，获得了388,197个单核苷酸多态性（SNPs）和6164个简单重复序列（SSR），这些多态性位点可以作为今后更丰富、更有价值的SSR分子标记。本研究结果有助于更好地了解BaP对珊瑚胚胎的毒性作用机制，对今后珊瑚礁生态系统的保护和恢复也具有重要意义。(C)2018爱思唯尔有限公司版权所有
Coral embryos are a critical and sensitive period for the early growth and development of coral. Benzo(a) pyrene (BaP) is widely distributed in the ocean and has strong toxicity, but there is little information on the toxic effects to coral embryos exposed to this widespread environmental contaminant. Thus, in this study, we utilized the Illumina Hiseg(TM) 4000 platform to explore the gene response of Acropora hyacinthus embryos under the BaP stress. A total of 130,042 Unigenes were obtained and analyzed, and approximately 37.67% of those matched with sequences from four different species. In total, 2606 Unigenes were up-regulated, and 3872 Unigenes were down-regulated. After Gene Ontology (GO) annotation, the results show that the "cellular process" and "metabolic process" were leading in the category of biological processes, which the "binding" and "catalytic activity" were the most abundant subcategories in molecular function. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the most differentially expressed genes (DEGs) were enriched, as well as down-regulated in the pathways of oxidative phosphorylation, metabolism of xenobiotics, immune-related genes, apoptosis and human disease genes. At the same time, 388,197 of Single-nucleotide Polymorphisms (SNPs) and 6164 of Simple Sequence Repeats (SSRs) were obtained, which can be served as the richer and more valuable SSRs molecular markers in the future. The results of this study can help to better understand the toxicological mechanism of coral embryo exposed to BaP, and it is also essential for the protection and restoration of coral reef ecosystem in the future. (C) 2018 Elsevier Ltd. All rights reserved.