A rapid method for quantitatively estimating metabolites in human plasma in the absence of synthetic standards using a combination of liquid chromatography/mass spectrometry and radiometric detection
A rapid method for quantitatively estimating metabolites in human plasma in the absence of synthetic standards using a combination of liquid chromatography/mass spectrometry and radiometric detection
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DOI：
10.1002/rcm.2863
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Neiss, Thomas G.
中科院分区：
文献类型：
--
作者：
Yu, ChungPing;Chen, Cathy L.;Neiss, Thomas G.
已经开发出一种在缺乏合成化学标准的情况下估计人血浆中药物相关代谢物水平的方法。该方法采用高效液相色谱/质谱（LC/MS）与辐射检测相结合。来自临床前体外和体内基质的生物来源的 [C-14] 代谢物用作 [C-14] 代谢物标准品，并根据相应的放射性计算它们在基质中的浓度。通过确定血浆和 [C-14] 代谢物标准品之间代谢物的相对 MS 响应，并使用计算出的代谢物标准品浓度作为校准物，可以估计人血浆样品中药物相关代谢物的量。使用估算人血浆中代谢物的示例来证明该方法的实用性。版权所有 (c) 2007 John Wiley & Sons, Ltd.
An approach to estimating the levels of drug-related metabolites in human plasma in the absence of synthesized chemical standards has been developed. High-performance liquid chromatography/ mass spectrometry (LC/MS) in combination with radiometric detection was used in this method. Biologically derived [C-14] metabolites from preclinical in vitro and in vivo matrices are used as [C-14] metabolite standards and their concentrations in matrices are calculated based on the corresponding radioactivity. The amount of drug-related metabolites in human plasma samples can be estimated by determining relative MS responses of metabolites between plasma and [C-14] metabolite standards, and using the calculated concentrations of metabolite standards as calibrants. An example for the estimation of metabolites in human plasma was used to demonstrate the utility of this methodology. Copyright (c) 2007 John Wiley & Sons, Ltd.