Engineered Peptide Repairs Defective Adhesive-Dentin Interface.
Engineered Peptide Repairs Defective Adhesive-Dentin Interface.
复制标题
工程肽修复有缺陷的粘合剂-牙本质界面。
DOI：
10.1002/mame.201600487
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发表时间：
2017
影响因子：
3.9
通讯作者：
Tamerler,Candan
中科院分区：
文献类型：
--
作者：
Ye,Qiang;Spencer,Paulette;Yuca,Esra;Tamerler,Candan
复合材料修复体的失败主要是由于牙齿-复合材料界面的反复腐蚀。在这个界面上，粘合剂及其与牙本质的结合是修复牙齿和口腔环境之间的屏障。在体内，在粘接剂/牙本质（a/d）界面形成的键的降解遵循一系列事件，导致复合修复体的减弱。在这里，开发了一种基于肽的方法来矿化a/d界面上的缺陷牙本质基质。具有在牙本质上自组装和诱导磷酸钙再矿化的固有能力的肽锚定在界面上。使用微拉曼光谱和荧光显微镜分析粘合剂、胶原蛋白和矿物质的分布。结果表明，缺牙本质基质的再矿化作用在整个界面中实现，且矿物分布均匀。这里展示的基于肽的再矿化可以成为设计集成生物材料-组织界面的使能技术。
Failure of dental composite restorations is primarily due to recurrent decay at the tooth–composite interface. At this interface, the adhesive and its bond with dentin is the barrier between the restored tooth and the oral environment. In vivo degradation of the bond formed at the adhesive/dentin (a/d) interface follows a cascade of events leading to weakening of the composite restoration. Here, a peptide‐based approach is developed to mineralize deficient dentin matrices at the a/d interface. Peptides that have an inherent capacity to self‐assemble on dentin and to induce calcium–phosphate remineralization are anchored at the interface. Distribution of adhesive, collagen, and mineral is analyzed using micro‐Raman spectroscopy and fluorescence microscopy. The analysis demonstrates remineralization of the deficient dentin matrices achieved throughout the interface with homogeneous distribution of mineral. The peptide‐based remineralization demonstrated here can be an enabling technology to design integrated biomaterial–tissue interfaces.