ALLELIC ASSOCIATION OF HUMAN DOPAMINE-D2 RECEPTOR GENE IN ALCOHOLISM
ALLELIC ASSOCIATION OF HUMAN DOPAMINE-D2 RECEPTOR GENE IN ALCOHOLISM
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DOI：
10.1001/jama.263.15.2055
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发表时间：
1990-04-18
影响因子：
120.7
通讯作者：
COHN, JB
中科院分区：
文献类型：
--
作者：
BLUM, K;NOBLE, EP;COHN, JB
在一个盲法实验中，我们报告了多巴胺D2受体基因在酒精中毒中的第一个等位基因关联。从70个酗酒者和非酗酒者的脑样品中，用限制性内切核酸酶消化DNA，并用含有人多巴胺D2受体基因（hD2 G1）的整个3 ″编码外显子、多聚腺苷酸化信号和约16.4个非编码3 ″序列的内切酶的克隆进行探测。在目前的样本中，多巴胺D2受体基因的A1等位基因的存在正确地分类了77%的酗酒者，而它的缺失分类了72%的非酗酒者。这种受体基因的多态性模式表明，一个基因，赋予易感性，至少一种形式的酒精中毒是位于q22-q23区域的染色体11。
In a blinded experiment, we report the first allelic association of the dopamine D2 receptor gene in alcoholism. From 70 brain samples of alcoholics and nonalcoholics, DNA was digested with restriction endonucleases and probed with a clone that contained the entire 3'' coding exon, the polyadenylation signal, and approximately 16.4 kilobases of noncoding 3''-sequence of the human dopamine D2 receptor gene (.lambda.hD2G1). In the present samples, the presence of the A1 allele of the dopamine D2 receptor gene correctly classified 77% of alcoholics, and its absence classified 72% of nonalcoholics. The polymorphic pattern of this receptor gene suggests that a gene that confers susceptibility to at least one form of alcoholism is located on the q22-q23 region of chromosome 11.