Faecal corticosterone concentrations indicate that separately housed male mice are not more stressed than group housed males
Faecal corticosterone concentrations indicate that separately housed male mice are not more stressed than group housed males
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DOI：
10.1016/j.physbeh.2005.11.013
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发表时间：
2006-03-30
影响因子：
2.9
通讯作者：
Hambly, C
中科院分区：
文献类型：
--
作者：
Hunt, C;Hambly, C
小鼠占所有实验动物的80%以上。这项研究调查了不同的住房条件如何影响压力水平，通过测量皮质酮水平，使用非侵入性粪便收集和行为。使用60只远系繁殖的MF 1雄性小鼠，在研究开始时将其分为5种不同的饲养条件，（A）单独饲养，地板面积490 cm？每个个体，（B）三只小鼠的组，每个个体的地板面积为163 cm（2），（C）三只小鼠的组，每个个体的地板面积为320 cm（2），（D）六只小鼠的组，每个个体的地板面积为160 cm（2），（E）六只小鼠的组，每个个体的地板面积为230 cm（2），具有额外的高度以允许视觉接触。所有饲养条件下的小鼠均提供基本的纸垫料和塑料房屋。这项研究的结果表明，单独饲养的小鼠在分离后随着时间的推移皮质酮水平降低，从14天开始达到最低水平。饲养在一起的6只小鼠的组随时间推移没有显示出差异。此外，不同饲养密度之间的皮质酮水平无显著差异，攻击或刻板行为无差异，表明该品系和性别的小鼠没有理想的群体密度。提供额外的富集笼导致组圈养小鼠的皮质酮水平显着下降，但单独圈养的小鼠保持不受影响，增加其富集水平。他们花了更多的时间在强化的笼子里睡觉，但刻板行为没有任何减少。对于分组饲养的小鼠，必须进行额外的富集以降低应激水平，从而提高其福利标准，而单独饲养的小鼠仅需要基本水平的富集，并且在进行测量之前应与其组分开至少2周。(c)2006年爱思唯尔公司All rights reserved.
Mice account for over 80% of all animals used in experimentation. This study investigated how different housing conditions affected stress levels by measuring both corticosterone levels, using non-invasive faecal collection, and behaviour. Sixty outbred MF1 male mice were used which were separated into five different housing conditions at the beginning of the study, (A) individually housed, floor area 490 cm? per individual, (B) groups of three mice, floor area 163 cm(2) per individual, (C) groups of three mice, floor area 320 cm(2) per individual, (D) groups of six mice, floor area 160 cm(2) per individual, (E) groups of six mice, floor area 230 cm(2) with extra height per individual to allow visual contact. Mice in all housing conditions were provided with a basic enrichment of paper bedding and a plastic house. The results from this study showed that singly housed mice reduced their corticosterone levels over time after separation reaching a minimum from 14 days onwards. Groups of 6 mice housed together showed no difference over time. Also there was no significant difference in corticosterone levels between the different housing densities, with no differences for aggression or stereotypical behaviour suggesting that there is no ideal group density for this strain and sex of mouse. Providing additional enrichment to the cages caused a significant decrease in corticosterone levels for group housed mice, but individually housed mice remained unaffected by increasing their enrichment level. They spent significantly more time sleeping in the enhanced cage but without any reduction in stereotypical behaviour. For group housed mice, additional enrichment should be mandatory to reduce stress levels and therefore increase their welfare standards, while singly housed mice required only basic levels of enrichment and should be separated from their group for a minimum of 2 weeks before measurements are taken. (c) 2006 Elsevier Inc. All rights reserved.