A bean common mosaic virus-resistance gene in the soybean variant V94-5152 was mapped to the Rsv4 locus conferring resistance to soybean mosaic virus
A bean common mosaic virus-resistance gene in the soybean variant V94-5152 was mapped to the Rsv4 locus conferring resistance to soybean mosaic virus
复制标题
大豆变异体 V94-5152 中的一个豆类普通花叶病毒抗性基因被定位到赋予对大豆花叶病毒抗性的 Rsv4 位点
DOI：
10.1007/s00122-021-03829-8
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发表时间：
2021-05-16
影响因子：
5.4
通讯作者：
Wang, Bin
中科院分区：
文献类型：
--
作者：
Liu, Xue-Ting;Wu, Xiao-Yi;Wang, Bin
在大豆变异体V94-5152中，一个BCMV抗性基因被定位到SMV抗性Rsv4位点附近，这表明V94-5152可能依赖Rsv4位点同时抵抗SMV和BCMV。大豆花叶病毒（Soybean mosaic virus， SMV）和大豆普通花叶病毒（Bean common mosaic virus， BCMV）都能诱发大豆花叶病，但迄今为止对大豆抗BCMV的研究很少。本研究将抗BCMV和SMV的大豆变异体V94-5152与易感品种Williams 82杂交，定位抗性基因。用BCMV分离物HZZB011接种292例F-2个体，分离比为3耐：1感，表明V94-5152对BCMV-HZZB011具有单显性抗性基因。群体分离分析（BSA）表明，该抗性基因与2号染色体上的SSR标记BARCSOYSSR_02_0617密切相关。对292个F-2个体相邻SSR标记进行基因分型，绘制了遗传连锁图谱，结果表明，bcmv抗性基因位于BARCSOYSSR_02_0617下游0.2 cM处。对该标记周围的10个候选基因（Glyma02g121300 ~ Glyma02g122200）进行扩增和测序，发现4个基因含有非同义变化或索引。此外，该位置靠近最近克隆的抗smv的Rsv4位点。通过从栽培和野生大豆材料中获得10多个Rsv4基因座序列，我们进一步研究了该病毒抗性基因座的变异和进化模式。很明显，阳性选择作用于该位点，其中一个关键氨基酸（R55P）的变化是所有抗性大豆共有的。
Key message In the soybean variant V94-5152, a BCMV-resistance gene was mapped near to the region of SMV-resistance Rsv4 locus, raising a possibility that V94-5152 may rely on Rsv4 locus to resist against both SMV and BCMV. Both Soybean mosaic virus (SMV) and Bean common mosaic virus (BCMV) can induce soybean mosaic diseases, but few studies have explored soybean resistance against BCMV so far. In this study, V94-5152, a soybean variant resistant to BCMV and SMV, was crossed with a susceptible cultivar, Williams 82 to map the resistance gene. By inoculating 292 F-2 individuals with a BCMV isolate HZZB011, a segregation ratio of 3 resistant: 1 susceptible was observed, suggesting that V94-5152 possesses a single-dominant resistance gene against BCMV-HZZB011. Bulk segregation analysis (BSA) then revealed that the resistance gene is closely linked to BARCSOYSSR_02_0617, a simple sequence repeat (SSR) marker on chromosome 2. Genotyping neighboring SSR markers among the 292 F-2 individuals enabled us to draw a genetic linkage map, which indicated that the BCMV-resistance gene is located 0.2 cM downstream of BARCSOYSSR_02_0617. Amplification and sequencing ten candidate genes (Glyma02g121300 to Glyma02g122200) around this marker then revealed four genes containing nonsynonymous changes or indels. Also, this location is near to the recently cloned SMV-resistance Rsv4 locus from the cultivar Peking. By obtaining ten more sequences of Rsv4 locus from cultivated and wild soybean materials, we further investigated the variation and evolutionary patterns of this virus-resistance locus. It was evident that positive selections had been acting on this locus, with one critical amino acid change (R55P) shared by all resistance soybeans tested.