CHARACTERIZATION OF RAINBOW-TROUT CDNAS ENCODING A SECRETED AND MEMBRANE-BOUND IG HEAVY-CHAIN AND THE GENOMIC INTRON UPSTREAM OF THE 1ST CONSTANT EXON
CHARACTERIZATION OF RAINBOW-TROUT CDNAS ENCODING A SECRETED AND MEMBRANE-BOUND IG HEAVY-CHAIN AND THE GENOMIC INTRON UPSTREAM OF THE 1ST CONSTANT EXON
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DOI：
10.1016/0161-5890(93)90075-m
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发表时间：
1993-05-01
影响因子：
3.6
通讯作者：
PILSTROM, L
中科院分区：
文献类型：
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作者：
LEE, MA;BENGTEN, E;PILSTROM, L
报道了两种不同的虹鳟鱼 cDNA 序列，编码重链分泌型 Ig (Hs) 和膜结合重链 Ig (Hm) 的一部分。这些序列与编码其他硬骨鱼 Ig 重链 (IgH) 的序列最为相似。与其他硬骨鱼的 Hm 一样，虹鳟鱼的 Hm 是由第三恒定外显子 (CH3) 的 3' 端与编码膜结合结构域的序列剪接而产生的。对虹鳟鱼 IgH 基因组克隆的分析表明，与 cDNA 中观察到的不同，连接重链 (JH) 片段位于 CH1 外显子 5' 825 bp 处。该序列还包含可能的增强子样和八聚体样基序。
Two different rainbow trout cDNA sequences encoding a heavy chain secreted Ig (Hs) and a part of a membrane-bound heavy chain Ig (Hm) are reported. The sequences were most similar to those encoding the Ig heavy chains (IgH) of other teleost fish. As in the Hm of the other teleost fish the rainbow trout Hm results from the splicing of the 3' end of the third constant exon (CH3) to the sequence encoding the membrane-bound domain. Analysis of a rainbow trout IgH genomic clone revealed that a joining heavy chain (JH) segment, different to the one observed in the cDNA, is located 825 bp 5' of the CH1 exon. The sequence also contains possible enhancer-like and octamer-like motifs.