Genetic and neuronal regulation of sleep by neuropeptide VF.
Genetic and neuronal regulation of sleep by neuropeptide VF.
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DOI：
10.7554/elife.25727
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发表时间：
2017-11-06
期刊：
影响因子：
7.7
通讯作者：
Prober DA
中科院分区：
文献类型：
--
作者：
Lee DA;Andreev A;Truong TV;Chen A;Hill AJ;Oikonomou G;Pham U;Hong YK;Tran S;Glass L;Sapin V;Engle J;Fraser SE;Prober DA
睡眠是一种必要的、在系统发育上保守的行为状态，但目前尚不清楚在无脊椎动物中发现的基因在多大程度上也能调节脊椎动物的睡眠。RFamide相关的神经肽已被证明可以促进无脊椎动物的睡眠，在这里，我们报道了脊椎动物下丘脑RFamide神经肽VF （NPVF）调节斑马鱼（一种昼行性脊椎动物）的睡眠。我们发现NPVF信号传导和表达NPVF的神经元对于促进睡眠都是必要和充分的，NPVF前原蛋白衍生的成熟肽以协同方式促进睡眠，并且刺激表达NPVF的神经元诱导与正常睡眠一致的神经元活动水平。这些结果表明，NPVF信号传导和NPVF表达神经元是一种新的脊椎动物睡眠促进系统，并表明RFamide神经肽参与了睡眠控制的一个古老而核心的方面。
Sleep is an essential and phylogenetically conserved behavioral state, but it remains unclear to what extent genes identified in invertebrates also regulate vertebrate sleep. RFamide-related neuropeptides have been shown to promote invertebrate sleep, and here we report that the vertebrate hypothalamic RFamide neuropeptide VF (NPVF) regulates sleep in the zebrafish, a diurnal vertebrate. We found that NPVF signaling and npvf-expressing neurons are both necessary and sufficient to promote sleep, that mature peptides derived from the NPVF preproprotein promote sleep in a synergistic manner, and that stimulation of npvf-expressing neurons induces neuronal activity levels consistent with normal sleep. These results identify NPVF signaling and npvf-expressing neurons as a novel vertebrate sleep-promoting system and suggest that RFamide neuropeptides participate in an ancient and central aspect of sleep control.