Assessment of Antibodies Induced by Multivalent Transmission-Blocking Malaria Vaccines.
Assessment of Antibodies Induced by Multivalent Transmission-Blocking Malaria Vaccines.
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评估由多价传播阻断疟疾疫苗诱导的抗体。
DOI：
10.3389/fimmu.2017.01998
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发表时间：
2017
影响因子：
7.3
通讯作者：
Biswas S
中科院分区：
文献类型：
--
作者：
Menon V;Kapulu MC;Taylor I;Jewell K;Li Y;Hill F;Long CA;Miura K;Biswas S
阻断疟疾传播的疫苗将是实现消灭和根除疟疾的一个重要工具。通过使用黑猩猩腺病毒血清型63和改良的安卡拉牛痘病毒病毒载体疫苗，我们研究了将两种抗原并入一种疫苗是否会导致比一种抗原更高的传播减少活性。我们证明，当Pfs 25与其他抗原Pfs 28或Pfs 230 C同时作为混合疫苗或作为双抗原疫苗共表达时，小鼠对每种抗原的抗体应答与单抗原疫苗相当，没有免疫干扰。然而，我们发现双抗原疫苗的传播减少活性（功能活性）不是累加的。双抗原疫苗通常仅引起与单抗原疫苗相似的传播减少活性，并且在一种情况下具有较低的传播减少活性。我们发现，尽管缺乏双抗原疫苗的免疫干扰，但它们在阻断疟疾传播方面仍然不如Pfs 25-IMX 313有效，Pfs 25-IMX 313是目前病毒载体疫苗的主要候选者。Pfs 25-顶X 313引起与双抗原疫苗相似质量的抗体，但更高的抗体滴度。
A malaria transmission-blocking vaccine would be a critical tool in achieving malaria elimination and eradication. By using chimpanzee adenovirus serotype 63 and modified vaccinia virus Ankara viral vectored vaccines, we investigated whether incorporating two antigens into one vaccine would result in higher transmission-reducing activity than one antigen. We demonstrated that when Pfs25 was administered with other antigens Pfs28 or Pfs230C, either concurrently as a mixed vaccine or co-expressed as a dual-antigen vaccine, the antibody response in mice to each antigen was comparable to a monoantigen vaccine, without immunological interference. However, we found that the transmission-reducing activity (functional activity) of dual-antigen vaccines was not additive. Dual-antigen vaccines generally only elicited similar transmission-reducing activity to monoantigen vaccines and in one instance had lower transmission-reducing activity. We found that despite the lack of immunological interference of dual-antigen vaccines, they are still not as effective at blocking malaria transmission as Pfs25-IMX313, the current leading candidate for viral vectored vaccines. Pfs25-IMX313 elicited similar quality antibodies to dual-antigen vaccines, but higher antibody titers.