A 75 KD MEROZOITE SURFACE PROTEIN OF PLASMODIUM-FALCIPARUM WHICH IS RELATED TO THE 70 KD HEAT-SHOCK PROTEINS
A 75 KD MEROZOITE SURFACE PROTEIN OF PLASMODIUM-FALCIPARUM WHICH IS RELATED TO THE 70 KD HEAT-SHOCK PROTEINS
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DOI：
10.1002/j.1460-2075.1987.tb04780.x
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发表时间：
1987-02-01
期刊：
影响因子：
11.4
通讯作者：
REESE, RT
中科院分区：
文献类型：
--
作者：
ARDESHIR, F;FLINT, JE;REESE, RT
人类恶性疟原虫裂殖子表面的蛋白质是宿主免疫反应的靶点。利用免疫电镜技术，用抗人cDNA克隆表达产物的抗血清，建立了恶性疟原虫p75蛋白裂殖子表面的定位。免疫沉淀从蛋白质提取物生物合成标记在不同时期的无性周期表明，p75是连续的，虽然ringstage寄生虫似乎合成大量。p75在8个恶性疟原虫分离株中是保守的，大小不变。编码p75部分的880 bp cDNA序列显示一个开放阅读框架，包含一个4个氨基酸的重复序列单位。预测的阅读框架是正确的，因为对应于重复区域的合成肽的抗血清在免疫印迹中识别p75。p75的序列与普遍存在的、高度保守的70 kd热休克蛋白家族的蛋白质序列同源，表明p75具有重要的生理功能。编码p75部分的cDNA片段与多个基因组片段杂交，其大小在来自9个恶性疟原虫株的DNA中相同，表明p75基因高度保守，可能是基因家族的一部分。
Proteins on the merozoite surface of the human malarial parasite Plasmodium falciparum are targets of the host''s immune response. The merozoite surface location of p75, a 75 kd P. falciparum protein, was established by immunoelectron microscopy using antisera raised to the expressed product of a cDNA clone. Immunoprecipitation from protein extracts biosynthetically labeled during different periods of the asexual cycle showing that p75 is made continuously, although ringstage parasites appear to synthesize large quantities. p75 is conserved and invariant in size in eight isolates of P. falciparum. The 880 bp cDNA sequence encoding part of p75 reveals one open reading frame containing a repetitive sequence unit of four amino acids. The predicted reading frame is correct since antisera to a synthetic peptide corresponding to the repetitive region recognize p75 in immunoblots. The sequence of p75 is homologous with the sequences of proteins from the ubiquitous, highly conserved family of 70 kd heatshock proteins, suggesting an important physiological function for p75. The cDNA fragment encoding part of p75 hybridizes with multiple genomic fragments, whose sizes are identical in DNA from nine P. falciparum strains, suggesting that the gene for p75 is well conserved and may be part of a gene family.