Expression profiling of genes coding for abundant proteins in the alkenone body of marine haptophyte alga Tisochrysis lutea
Expression profiling of genes coding for abundant proteins in the alkenone body of marine haptophyte alga Tisochrysis lutea
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海洋固定藻类黄金藻烯酮体中丰富蛋白质编码基因的表达谱
DOI：
10.1186/s12866-019-1430-x
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发表时间：
2019-03
期刊：
影响因子：
4.2
通讯作者：
Shi Qing
中科院分区：
文献类型：
--
作者：
Shi Qing
背景：产烯酮的海洋附着菌黄组织鞭金藻脂体中含有丰富的蛋白质。这些蛋白质的基因表达模式进行了研究，以更好地了解它们在烯酮生物合成中的作用。为此，T。结果：缺氮培养后，叶黄素细胞的烯酮体（alkenone body，AB）体积和烯酮含量均显著增加。在培养期间，研究了编码鉴定的蛋白质V-ATP酶亚基VA、V-ATP酶亚基Vd、假设蛋白质EMIHUDRAFT_465，517、球石蚧相关蛋白-1、环阿屯醇-c-24-甲基转移酶1样和含SPFH结构域蛋白的基因的相对mRNA水平。RT-PCR结果显示，在氮充足到氮缺乏的过渡期，除编码SPFH结构域蛋白的基因外，其他基因的表达均上调。其中，颗石蚧相关蛋白-1基因的表达上调了50-650倍。这些上调与缺氮培养基中增加的烯酮产量一致，表明这些蛋白质参与了T.结论：脂蛋白基因的表达分析表明，6个基因中的5个上调，因此可能编码与T. lutea中烯酮生物合成相关的已鉴定脂蛋白。lutea这些数据将有助于更好地了解烯酮代谢和工程，以改善生物燃料生产的T。lutea
Background:Several abundant proteins have been identified in lipid body of an alkenone-producing marine haptophyte alga Tisochrysis lutea. The gene expression patterns of these proteins were investigated to better understand their roles in alkenone biosynthesis. For this purpose, T. lutea was first cultured in nitrogen-sufficient medium for biomass production and then shifted to nitrogen-deprived medium to induce lipid body formation.Results:There were remarkable increases in the volume of alkenone body (AB) and alkenone content in the alga after they were exposed to nitrogen depletion medium. Relative mRNA levels of the genes coding for the identified proteins V-ATPase subunit VA, V-ATPase subunit Vd, hypothetical protein EMIHUDRAFT_465,517, coccolith scale associated protein-1, cycloartenol-c-24-methyltransferase 1-like and SPFH domain-containing protein were investigated over the culture period. RT-PCR data showed that the expression of all these genes except the gene coding for SPFH domain-containing protein was up-regulated during the transition period from nitrogen-sufficient to nitrogen-deficient medium. Among them, the expression of the coccolith scale associated protein-1 gene was up-regulated 50-650 folds. These up-regulations were consistent with the increased alkenone production in nitrogen-deprived medium, suggesting that these proteins are involved in alkenone biosynthesis in T. lutea.Conclusions:Expression analysis of the lipoprotein genes suggests that five out of the six genes are up-regulated and are therefore likely to code for the identified lipoproteins associated with alkenone biosynthesis in T. lutea. These data would help better understand alkenone metabolism and engineer for improved biofuel production in T. lutea.
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影响因子：
3.4
作者：
Shi, Qing;Araie, Hiroya;Bakku, Ranjith Kumar;Fukao, Yoichiro;Rakwal, Randeep;Suzuki, Iwane;Shiraiwa, Yoshihiro
通讯作者：
Shiraiwa, Yoshihiro
影响因子：
64.8
作者：
Peters, C;Bayer, MJ;Mayer, A
通讯作者：
Mayer, A
DOI：
10.1007/978-3-319-25979-6_1
发表时间：
2016-01-01
期刊：
LIPIDS IN PLANT AND ALGAE DEVELOPMENT
影响因子：
--
作者：
Li-Beisson, Yonghua;Nakamura, Yuki;Harwood, John
通讯作者：
Harwood, John
DOI：
10.1080/00071618400650221
发表时间：
1984-01-01
期刊：
BRITISH PHYCOLOGICAL JOURNAL
影响因子：
--
作者：
MARLOWE, IT;GREEN, JC;COURSE, PA
通讯作者：
COURSE, PA
DOI：
10.1016/b978-0-08-101023-5.00010-8
发表时间：
2017
期刊：
--
影响因子：
--
作者：
L. Gouveia;A. C. Oliveira;R. Congestri;L. Bruno;A. T. Soares;R. S. Menezes;N. Filho;I. Tzovenis
通讯作者：
L. Gouveia;A. C. Oliveira;R. Congestri;L. Bruno;A. T. Soares;R. S. Menezes;N. Filho;I. Tzovenis