Application of dual-focus fluorescence correlation spectroscopy to microfluidic flow-velocity measurement
Application of dual-focus fluorescence correlation spectroscopy to microfluidic flow-velocity measurement
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DOI：
10.1039/b924594d
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Enderlein, Joerg
中科院分区：
文献类型：
--
作者：
Arbour, Tyler J.;Enderlein, Joerg
有几种方法可以测量和绘制微流体装置中的流体速度，这对于理解微纳米尺度上的特性至关重要。荧光相关光谱（FCS）是一种传统的方法，利用其测量分子扩散系数的能力。然而，过去十年的一些报告表明，FCS也可以成功地用于测量微流体中的精确流量，具有非常高的空间分辨率，使其成为其他常见流量测量方法的竞争性替代方案。2007年，我们推出了传统FCS的改进版本，克服了许多困扰标准技术的伪影。在这里，我们展示了这种称为双焦点FCS的方法的优点如何扩展到流量测量。为此，我们测量了沿方孔微流控通道横截面的流速流动剖面，并将结果与理论预测结果进行了比较。
Several methods exist to measure and map fluid velocities in microfluidic devices, which are vital to understanding properties on the micro-and nano-scale. Fluorescence correlation spectroscopy (FCS) is a method traditionally exploited for its ability to measure molecular diffusion coefficients. However, several reports during the past decade have shown that FCS can also be successfully used to measure precise flow rates in microfluidics with very high spatial resolution, making it a competitive alternative to other common flow-measurement methods. In 2007 we introduced a modified version of conventional FCS that overcomes many of the artifacts troubling the standard technique. Here we show how the advantages of this method, called dual-focus FCS, extend to flow measurements. To do so, we have measured the velocity flow profile along the cross-section of a square-bore microfluidic channel and compared the result to the theoretical prediction.