Signal transduction through ion channels associated with excitatory amino acid receptors.
Signal transduction through ion channels associated with excitatory amino acid receptors.
复制标题
通过与兴奋性氨基酸受体相关的离子通道进行信号转导。
DOI：
10.1016/s0076-6879(99)94024-7
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发表时间：
1999
影响因子：
--
通讯作者：
Y. Yoneda
中科院分区：
文献类型：
--
作者：
K. Ogita;Y. Yoneda
兴奋性氨基酸（EAA）受体根据其在哺乳动物中枢神经系统（CNS）中的信号转导过程目前分为两大类，包括亲离子型和亲代谢型。代谢型EAA受体家族迄今为止包括至少八个不同的亚基（mGluR 1 -8），并且基于结构和信号转导系统的差异被细分为三组。另一方面，根据对N-甲基-D-天冬氨酸（NMDA）的不同敏感性，与阳离子通道相连的离子型EAA受体在生理学上被分为两大类。NMDA通道对Ca 2+具有高度渗透性，不同的离子通道与非NMDA受体偶联。本章讨论了受体结合试验，电泳迁移率变动试验（EMSA）和免疫印迹分析，用于评价从细胞膜到细胞核的信号转导。
Publisher Summary Excitatory amino acid (EAA) receptors are currently divided into two major categories according to their signal transduction processes in the mammalian central nervous system (CNS), including the ionotropic and metabotropic classes. The metabotropic EAA receptor family so far comprises at least eight different subunits (mGluR1-8) and is subclassified into three groups based on differences in structure and signal transduction systems. On the other hand, ionotropic EAA receptors linked to cation channels are pharmacologically and physiologically classified into two major categories according to differential sensitivities to N-methyl-D-aspartic acid (NMDA). The NMDA channel is highly permeable to Ca2+, with different ion channels coupled to non-NMDA receptors. This chapter discusses a receptor binding assay, an electrophoretic mobility shift assay (EMSA) and an immunoblotting analysis for evaluation of signal transduction from membranes to nuclei.