Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat.
Hepatoprotective effect of food preservatives (butylated hydroxyanisole, butylated hydroxytoluene) on carbon tetrachloride-induced hepatotoxicity in rat.
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DOI：
10.1016/j.toxrep.2017.12.009
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发表时间：
2018
影响因子：
--
通讯作者：
Samanta S
中科院分区：
文献类型：
--
作者：
Dassarma B;Nandi DK;Gangopadhyay S;Samanta S
关于雄性白化病大鼠（Wister品系）肝毒性发生所用剂量（230 mg/kg体重/大鼠/天）的报告来自我们自己的既往研究，其他任何研究组从未使用过该剂量。本研究对活性氧介导的肝毒性和氧化应激特征进行了详细的研究，是以往研究的重要成果之一。加尔斯级合成食品防腐剂BHA、BHT对雄性白化病大鼠的肝毒性有一定的减轻作用，这在本研究中尚属首次报道。四氯化碳（CCl 4）是一种肝毒性物质，广泛用于研究实验动物的毒性机制。本研究旨在建立食品防腐抗氧化剂丁基羟基茴香醚和丁基羟基甲苯（BHA，BHT）与食物混合对四氯化碳（CCl 4）中毒（230 mg/ kg B wt/大鼠/d）大鼠肝脏的保护措施。检测血清谷草转氨酶（AST）、谷草转氨酶（ALT）、碱性磷酸酶（ALP）、胆红素含量、抗氧化酶（SOD、CAT、GPx）及脂质过氧化产物丙二醛（MDA）。结果表明，CCl_4处理后AST、ALT、ALP、胆红素分别升高121.16%、124.68%、122.41%、57.14%。抗氧化酶SOD（85.36%）、CAT（67.47%）、GPx（50.7%）活性显著下降，说明活性氧介导的毒性作用和BHA、BHT的预处理使这些酶的活性恢复。由于氧化应激，还观察到高水平的MDA含量和降低的GSH值。肝脏的抗氧化状态恢复与食品防腐剂（BHA，BHT）抗氧化剂治疗，这表明显着的保护作用，对四氯化碳诱导的肝毒性，并最终证实了组织病理学研究。
The report about dose (230 mg/kg body wt/rat/day) that had been used for development of hepatotoxicity in male albino rat (Wister strain) was taken from our own previous study and it had never been used by any other group of researchers. This study had shown the detailed results of ROS mediated hepatotoxicity and oxidative stress profiles which was one of the important reports among the previous findings. GARS grade synthetic food preservative BHA, BHT could be able to reduce the hepatotoxicity in male albino (Wister strain) rats and it is the first time report in our study. Carbon tetrachloride (CCl4), a hepatotoxic agent is widely used to study the toxic mechanisms in experimental animals. This study was carried out to establish the hepatoprotective measures of food preservative antioxidants butylated hydroxyanisole and butylated hydroxytolune (BHA, BHT) when mixed with food towards carbon tetrachloride (CCl4) intoxication (230 mg/ kg b wt/rat/day) in rat. Biochemical markers like serum glutamate pyruvate tranaminase (AST), serum glutamate oxaloacetate transaminase (ALT), alkaline phosphatase (ALP) and bilirubin content, antioxidant enzymes such as SOD, CAT, GPx, and malondialdehyde (MDA) as the end product of lipid peroxidanion were measured. The results had shown the elevated level of AST (121.16%), ALT (124.68%), ALP (122.41%) an, bilirubin content (57.14%) after CCl4 treatment. Marked decrease of activity of antioxidant enzymes such as SOD (85.36%), CAT (67.47%), GPx (50.7%) had indicated that the ROS mediated toxicity and pretreatment of BHA and BHT restored the activity of these enzymes. High level of MDA content with reduced GSH value was also observed due to oxidative stress. The hepatic antioxidant status was restored with the food preservative (BHA, BHT) antioxidant treatment which had indicated the significant protective effect against CCl4 induced hepatotoxicity and finally confirmed by histopathological studies.