The effect of ethanol on the passive Ca permeability of human red cell ghosts measured by means of arsenazo III.
The effect of ethanol on the passive Ca permeability of human red cell ghosts measured by means of arsenazo III.
复制标题
通过偶氮胂 III 测量乙醇对人红细胞血影被动 Ca 渗透性的影响。
DOI：
10.1016/0005-2736(85)90242-1
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Kilgore,P
中科院分区：
文献类型：
--
作者：
Yingst,DR;Polasek,PM;Kilgore,P
乙醇在0.76-2.40 M的范围内引起的钙渗透性的重新密封的人红细胞鬼，其中细胞内游离钙可以连续监测的钙生色团偶氮胂III的装置的质膜的立即增加。在一个给定的浓度的乙醇，钙渗透性显着增加几分钟后的鬼和乙醇的混合，并继续增加至少在未来30分钟。预孵育的鬼在乙醇中15，60和120分钟，然后再测量游离钙积累的速率，逐步增加了一个给定浓度的乙醇的效果。这些结果表明，给定浓度的乙醇的影响是浓度和暴露时间的复杂函数。乙醇在此浓度范围内的影响是完全可逆的。在这些实验中使用的重新密封的鬼耗尽ATP，以避免干扰的Ca泵和所有的实验进行与150 mM KCl的膜两侧，以尽量减少与Ca依赖性K通道的激活相关的体积或膜电位的变化。
Ethanol in the range of 0.76–2.40 M caused an immediate increase in the Ca permeability of the plasma membrane of resealed human red blood cell ghosts in which intracellular free Ca could be continuously monitored by means of the Ca chromophore arsenazo III. At a given concentration of ethanol, the Ca permeability increased markedly a few minutes following the mixing of the ghosts and the ethanol, and continued to increase over at least the next 30 min. Preincubating the ghosts in ethanol for 15, 60 and 120 min before measuring the rate of free Ca accumulation, progressively increased the effect of a given concentration of ethanol. These results indicate that the effect of a given concentration of ethanol is a complex function of concentration and exposure time. The effects of ethanol in this concentration range were completely reversible. The resealed ghosts used in these experiments were depleted of ATP to avoid interference from the Ca pump and all experiments were carried out with 150 mM KCl on both sides of the membrane to minimize changes in either the volume or membrane potential associated with activation of the Ca-dependent K channel.