Depth-specific optogenetic control in vivo with a scalable, high-density μLED neural probe.
Depth-specific optogenetic control in vivo with a scalable, high-density μLED neural probe.
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DOI：
10.1038/srep28381
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发表时间：
2016-06-23
影响因子：
4.6
通讯作者：
Mathieson K
中科院分区：
文献类型：
--
作者：
Scharf R;Tsunematsu T;McAlinden N;Dawson MD;Sakata S;Mathieson K
控制神经回路是揭示神经活动和行为之间因果关系的有效方法。光遗传学已被神经科学界广泛采用，因为它提供了毫秒级精度的细胞类型特异性扰动。然而，这些研究需要以具有细胞尺度分辨率的复杂模式进行光传输，同时覆盖体内深度的大量组织。在这里，我们描述了一种新型高密度硅基微型发光二极管 (μLED) 阵列，该阵列由多达 96 个直径为 25μm 的 μLED 组成，发射波长为 450nm，峰值辐照度为 400mW/mm2。 100μm 的宽度、逐渐变细至 1μm 点和 40μm 的厚度有助于最大限度地减少插入过程中的组织损伤。热特性允许一组光遗传学操作机制，平均温度升高约 0.5°C。我们证明了小鼠体内新皮质神经元的深度依赖性激活，为精确操纵神经活动提供了一种廉价的新工具。
Controlling neural circuits is a powerful approach to uncover a causal link between neural activity and behaviour. Optogenetics has been widely adopted by the neuroscience community as it offers cell-type-specific perturbation with millisecond precision. However, these studies require light delivery in complex patterns with cellular-scale resolution, while covering a large volume of tissue at depth in vivo. Here we describe a novel high-density silicon-based microscale light-emitting diode (μLED) array, consisting of up to ninety-six 25 μm-diameter μLEDs emitting at a wavelength of 450 nm with a peak irradiance of 400 mW/mm2. A width of 100 μm, tapering to a 1 μm point, and a 40 μm thickness help minimise tissue damage during insertion. Thermal properties permit a set of optogenetic operating regimes, with ~0.5 °C average temperature increase. We demonstrate depth-dependent activation of mouse neocortical neurons in vivo, offering an inexpensive novel tool for the precise manipulation of neural activity.