Insertional mutagenesis and characterization of a polyketide synthase gene (PKS1) required for melanin biosynthesis in Bipolaris oryzae
Insertional mutagenesis and characterization of a polyketide synthase gene (PKS1) required for melanin biosynthesis in Bipolaris oryzae
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DOI：
10.1016/j.femsle.2004.07.007
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发表时间：
2004-09-01
影响因子：
2.1
通讯作者：
Honda, Y
中科院分区：
文献类型：
--
作者：
Moriwaki, A;Kihara, J;Honda, Y
利用限制性内切酶介导整合技术，分离到植物病原真菌米叶双星（Bipolaris oryzae）黑色素生物合成途径中的聚酮合成酶基因PKS1。序列分析表明，PKS1编码一个含有2155个氨基酸的推测蛋白，与其他真菌聚酮合成酶有显著的相似性。PKS1基因的靶向破坏表明，它是米芽孢杆菌黑色素生物合成所必需的。Northern blot分析显示，近紫外辐射（300-400 nm）特异性增强了PKS1转录本，其时间转录模式与B. oryzae黑色素生物合成途径中涉及的THR1和SCD1基因相似。(C) 2004年由Elsevier B.V.代表欧洲微生物学会联合会出版。
A polyketide synthase gene named PKS1, involved in the melanin biosynthesis pathway of the phytopathogenic fungus Bipolaris oryzae, was isolated using restriction enzyme-mediated integration. Sequence analysis showed that the PKS1 encodes a putative protein that has 2155 amino acids and significant similarity to other fungal polyketide synthases. Targeted disruption of the PKS1 gene showed that it is necessary for melanin biosynthesis in B. oryzae. Northern blot analysis showed that PKS1 transcripts were specifically enhanced by near-ultraviolet radiation (300-400 nm) and that its temporal transcriptional patterns were similar to those of THR1 and SCD1 genes involved in the melanin biosynthesis pathway of B. oryzae. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.