Estrogen increases glucose-induced insulin secretion from mouse pancreatic islets cultured in a prolonged high glucose condition.
Estrogen increases glucose-induced insulin secretion from mouse pancreatic islets cultured in a prolonged high glucose condition.
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发表时间：
2007-05
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通讯作者：
Suwattanee Kooptiwut;Namoiy Semprasert;S. Chearskul
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作者：
Suwattanee Kooptiwut;Namoiy Semprasert;S. Chearskul
背景众所周知，男性比女性更容易患2型糖尿病。雌激素对胰岛具有保护作用，使其免受淀粉样蛋白等有毒物质的侵害。雌激素在高糖环境下对胰岛的保护作用尚不清楚。目的在卵巢切除动物中给予雌激素显示出对2型糖尿病的保护作用。本研究旨在确定雌激素对高糖长期培养后胰岛功能的直接影响。材料和方法将雌激素（10-1 M乙醇溶液）与小鼠胰岛在正常葡萄糖培养基（11.1mM）中共培养3小时或与正常和高葡萄糖培养基（40 mM）共培养10天。结果在正常葡萄糖培养的胰岛细胞中，雌激素可增加3小时和10天的胰岛素分泌。胰岛长时间暴露于高葡萄糖会导致葡萄糖诱导的胰岛素分泌受损，而10（-5）M雌激素的存在可部分消除这种作用。结论雌激素对长期高糖损伤的小鼠胰岛分泌胰岛素有直接的促进作用。
BACKGROUND It is known that males are more susceptible to develop type 2 diabetes than females. Estrogen has a protective effect on pancreatic islet against toxic agent such as amyloid. The role of estrogen in protection pancreatic islet against high glucose is still unknown. OBJECTIVE Administration of estrogen in an ovariectomised animal shows a protective effect against type 2 diabetes. The present study aimed to determine the direct effect of estrogen on the islet function after prolonged culture in high glucose. MATERIAL AND METHOD Estrogen (10-1 M in ethanol) was co-cultured with mouse pancreatic islets in normal glucose medium (11.1 mM) for 3 hours or with normal and high glucose medium (40 mM) for 10 days. RESULTS Estrogen increased glucose-induced insulin secretion in islet culture in normal glucose medium for both 3-hour and 10-day culture. Prolonged exposure of pancreatic islet to high glucose generated impaired glucose-induced insulin secretion, which was partially abrogated by the presence of 10(-5) M estrogen. CONCLUSION These results indicated a direct effect of estrogen on improving insulin secretion from mouse pancreatic islets that has been impaired by prolonged exposure to high glucose.