Analysis of Glycosaminoglycan from Diabetic and Normal Chinese Hamster Cells
Analysis of Glycosaminoglycan from Diabetic and Normal Chinese Hamster Cells
复制标题
糖尿病和正常中国仓鼠细胞的糖胺聚糖分析
作者：
L. Ginsberg;B. Wyse;A. Chang
在含35SO4和3h -氨基葡萄糖的培养基中培养糖尿病肾细胞株和正常肾细胞株。通过酶和电泳程序从培养基、胰蛋白酶和细胞颗粒中提取和分析糖胺聚糖（GAG）。与正常人相比，糖尿病GAGs的所有三个部分的透明质酸含量都显著增加。此外，糖尿病细胞系中硫酸肝素含量增加，硫酸软骨素含量减少。这些数据表明，其他人在糖尿病肾脏中看到的体内GAG类型和数量的变化也可能在培养细胞中看到。
Diabetic and normal cell lines from Chinese hamster kidneys were cultured in media containing 35SO4 and 3H-glucosamine. Glycosaminoglycans (GAG) were extracted and analyzed from the media, trypsin, and cell pellet by enzymatic and electrophoretic procedures. Significant increases in the hyaluronic acid content were noted in all three fractions of diabetic GAGs when compared with normals. In addition, an increased heparan sulfate content and decreased chondroitin sulfate amounts were noted in diabetic cell lines. These data suggest that in vivo changes in GAG types and amounts in diabetic kidneys seen by others may also be seen in cultured cells.