Effects of Elastic Waves at Several Frequencies on Biofilm Formation in Circulating Types of Laboratory Biofilm Reactors
Effects of Elastic Waves at Several Frequencies on Biofilm Formation in Circulating Types of Laboratory Biofilm Reactors
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几种频率下的弹性波对循环型实验室生物膜反应器中生物膜形成的影响
DOI：
10.1002/9781119543299.ch5
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Yoshimitsu Mizunoe
中科院分区：
文献类型：
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作者：
Hideyuki Kanematsu;Shogo Maeda;Dana M. Barry;Senshin Umeki;Kazuyuki Tohji;Nobumitsu Hirai;Akiko Ogawa;Takeshi Kogo;Hajime Ikegai;Yoshimitsu Mizunoe
本章重点研究了循环式实验室生物膜反应器（LBR）中应用弹性波与生物膜形成之间的相关性。在实验中，将几个金属试件插入LBR中，并在一定时间内对其施加弹性波。另一种测量方法是使用结晶紫试剂的染色试验。这种化学物质可以染色细菌本身和EPS。将样品浸入0.1%结晶紫溶液中30分钟。中间颜色是指最高和最低之间的高度，它们根据高度而变化。当施加超声波时，观察到轻微的海岛图案。由于光学显微镜观察是局部测量，结果表明在铝表面的某些地方仍然形成生物膜。镀铬试样不受声波的影响。在有声波和没有声波的情况下，紫色的程度没有变化。对于玻璃试样也观察到相同的趋势。
This chapter focuses on the correlation between applied elastic waves and biofilm formation in a circulating type of laboratory biofilm reactor (LBR). Several metallic specimens were inserted into the LBR and elastic waves were applied to them for a certain period of time in an experiment. The other method of measurement was the staining test using crystal violet agents. This chemical can stain bacteria themselves and also EPS. Specimens were immersed in 0.1% crystal violet solutions for 30 minutes. The intermediate colors refer to heights between the highest and lowest and they change according to the height. The slight sea‐island patterns were observed when the ultrasonic waves were applied. Since the optical microscopic observation was a local measurement, the results suggest that biofilm still formed at some places on the aluminum surface. Chromium plated specimens were not affected by sonic waves. The extent of violet colors did not change in the case with and without sonic waves. The same tendency was also observed for the glass specimens.