IGF2 mRNA binding protein 3 (IMP3) mediated regulation of transcriptome and translatome in glioma cells
IGF2 mRNA binding protein 3 (IMP3) mediated regulation of transcriptome and translatome in glioma cells
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DOI：
10.1080/15384047.2017.1323601
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发表时间：
2018-01-01
影响因子：
3.6
通讯作者：
Somasundaram, Kumaravel
中科院分区：
文献类型：
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作者：
Bhargava, Shruti;Patil, Vikas;Somasundaram, Kumaravel
RNA结合蛋白介导细胞转录组和翻译组水平的全局调节。我们分别使用 IMP3 沉默的胶质瘤细胞的总细胞 RNA 和重聚核糖体衍生的 RNA 进行微阵列，研究了转录组和翻译组中 IMP3 调节的全局水平表达变化。鉴定了转录组水平（n = 2388）和翻译组水平（n = 479）的差异调节转录物。此外，根据 IMP3 结合位点的存在，这些转录物被分为直接和间接靶标。进一步的研究表明，转录组水平的直接靶点被发现与细胞周期相关的过程相关，而翻译组水平的直接靶点参与细胞凋亡相关途径。还研究了两个层面间接调节的可能机制。总的来说，我们的研究揭示了 IMP3 在神经胶质瘤细胞中施加的多水平基因表达调控。
RNA binding proteins mediate global regulation at the level of transcriptome and translatome of a cell. We studied the global level expression changes regulated by IMP3 in transcriptome and translatome by performing microarray using total cellular RNA and heavy polysome derived RNA of IMP3 silenced glioma cells respectively. Differentially regulated transcripts at the transcriptome level (n = 2388) and at the level of translatome (n = 479) were identified. Further, these transcripts were classified as direct and indirect targets on the basis of presence of IMP3 binding site. Additional investigation revealed that direct targets at transcriptome level were found to be associated with processes related to cell cycle, whereas direct targets at the translatome level participated in apoptosis related pathways. Probable mechanism of indirect regulation at both the levels is also investigated. Collectively, our study reveals multi-level gene expression regulation imposed by IMP3 in glioma cells.