A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration.
A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration.
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MSC亲和肽修饰的脱矿骨基质颗粒与壳聚糖水凝胶复合支架用于软骨再生
DOI：
10.1038/srep17802
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发表时间：
2015-12-03
影响因子：
4.6
通讯作者：
Ao Y
中科院分区：
文献类型：
--
作者：
Meng Q;Man Z;Dai L;Huang H;Zhang X;Hu X;Shao Z;Zhu J;Zhang J;Fu X;Duan X;Ao Y
关节软骨损伤仍然是一个重大挑战，因为软骨的内在愈合潜力差。基于干细胞的组织工程是软骨修复的有前途的技术。由于软骨缺陷通常在临床环境中不规则，因此具有可折磨能力的脚手架可以填充任何软骨缺陷的形状并与宿主软骨紧密整合。在这项研究中，我们构建了一个复合支架，结合了间充质干细胞（MSC）E7亲和肽修饰的非矿化骨基质（DBM）颗粒和壳聚糖（CS）水凝胶用于软骨工程。这种固体支持的复合支架表现出适当的孔隙度，提供了支持细胞粘附和增殖的3D微环境。细胞增殖和DNA含量分析表明，与CS或DBM/CS组相比，DBM-E7/CS支架促进了更好的大鼠骨髓衍生的MSC（BMMSC）生存。同时，DBM-E7/CS支架增加了基质产生，并提高了BMMSC在体外的软骨分化能力。此外，在体内植入四周后，与对照组相比，DBM-E7/CS组中的再生问题表现出半透明和类似软骨样结构，如大量观察，组织学检查和MATRIX STENT的评估所表明的那样。总体而言，DBM-E7/CS的功能复合支架是修复不规则形状的软骨缺陷的有前途选择。
Articular cartilage injury is still a significant challenge because of the poor intrinsic healing potential of cartilage. Stem cell-based tissue engineering is a promising technique for cartilage repair. As cartilage defects are usually irregular in clinical settings, scaffolds with moldability that can fill any shape of cartilage defects and closely integrate with the host cartilage are desirable. In this study, we constructed a composite scaffold combining mesenchymal stem cells (MSCs) E7 affinity peptide-modified demineralized bone matrix (DBM) particles and chitosan (CS) hydrogel for cartilage engineering. This solid-supported composite scaffold exhibited appropriate porosity, which provided a 3D microenvironment that supports cell adhesion and proliferation. Cell proliferation and DNA content analysis indicated that the DBM-E7/CS scaffold promoted better rat bone marrow-derived MSCs (BMMSCs) survival than the CS or DBM/CS groups. Meanwhile, the DBM-E7/CS scaffold increased matrix production and improved chondrogenic differentiation ability of BMMSCsin vitro. Furthermore, after implantationin vivofor four weeks, compared to those in control groups, the regenerated issue in the DBM-E7/CS group exhibited translucent and superior cartilage-like structures, as indicated by gross observation, histological examination and assessment of matrix staining. Overall, the functional composite scaffold of DBM-E7/CS is a promising option for repairing irregularly shaped cartilage defects.