Illumina MiSeq sequencing reveals microbial community in HA process for dyeing wastewater treatment fed with different co-substrates.
Illumina MiSeq sequencing reveals microbial community in HA process for dyeing wastewater treatment fed with different co-substrates.
复制标题
DOI：
10.1016/j.chemosphere.2018.03.025
复制
发表时间：
2018-06
期刊：
影响因子：
8.8
通讯作者：
Xuehui Xie;Na Liu;Jing Ping;Qingyun Zhang;Xiulin Zheng;Jianshe Liu
中科院分区：
文献类型：
--
作者：
Xuehui Xie;Na Liu;Jing Ping;Qingyun Zhang;Xiulin Zheng;Jianshe Liu
采用水解酸化（HA）反应器处理模拟印染废水。共底物包括淀粉、葡萄糖、蔗糖、酵母提取物（YE）和蛋白胨，它们被依次进料到HA反应器中以增强HA过程效果。研究了不同共基质条件下HA反应器的运行性能和HA工艺中微生物群落结构。结果表明，不同共基质对HA反应器的性能有不同的影响。以蔗糖为共基质时，HA反应器的脱色率和COD去除率最高，分别为50.64%和60.73%。碳源共基质淀粉、葡萄糖和蔗糖对HA反应器的脱色效果和COD去除率均优于氮源共基质YE和蛋白胨。通过Illumina MiSeq测序分析HA工艺中的微生物群落结构。结果表明，不同共基质对腐殖酸处理过程中微生物群落结构和多样性的影响不同。认为蔗糖能富集降解染料的Raoultella、Desulfovibrio、Tolumonas、Clostridium等菌种。蔗糖被认为是提高HA反应器性能的最佳共基质。本研究为深入了解不同共基质对HA反应器性能和HA工艺中微生物群落的影响提供了理论依据。
In present study, a hydrolysis acidification (HA) reactor was used for simulated dyeing wastewater treatment. Co-substrates included starch, glucose, sucrose, yeast extract (YE) and peptone were fed sequentially into the HA reactor to enhance the HA process effects. The performance of the HA reactor and the microbial community structure in HA process were investigated under different co-substrates conditions. Results showed that different co-substrates had different influences on the performance of HA reactor. The highest decolorization (50.64%) and COD removal rate (60.73%) of the HA reactor were obtained when sucrose was as the co-substrate. And it found that carbon co-substrates starch, glucose and sucrose exhibited better decolorization and higher COD removal efficiency of the HA reactor than the nitrogen co-substrates YE and peptone. Microbial community structure in the HA process was analyzed by Illumina MiSeq sequencing. Results revealed different co-substrates had different influences on the community structure and microbial diversity in HA process. It was considered that sucrose could enrich the species such asRaoultella,Desulfovibrio,Tolumonas,Clostridium, which might be capable of degrading the dyes. Sucrose was considered to be the best co-substrate of enhancing the HA reactor’s performance in this study. This work would provide deep insight into the influence of many different co-substrates on HA reactor performance and microbial communities in HA process.