Evidence that sensitivity to growth hormone (GH) is growth period and tissue type dependent:: Studies in GH-deficient lit/lit mice
Evidence that sensitivity to growth hormone (GH) is growth period and tissue type dependent:: Studies in GH-deficient lit/lit mice
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DOI：
10.1210/en.2002-0123
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发表时间：
2003-09-01
期刊：
影响因子：
4.8
通讯作者：
Mohan, S
中科院分区：
文献类型：
--
作者：
Kasukawa, Y;Baylink, DJ;Mohan, S
我们以前发现，生长激素缺乏引起的骨骼缺陷的大小在不同的生长时期有所不同。为了检验生长激素敏感性是生长期依赖性的假设，我们用生长激素处理生长激素缺乏的lit/lit小鼠，（4 mg/kg体重.d）或溶媒，在青春期前和青春期（d 7-34）、青春期（d 23-34）、青春期后（d 42-55），和成人（d 204-217）时期，并通过双能X线吸收测定法（DEXA）和外周定量计算机断层扫描评价GH对肌肉骨骼系统的影响。与溶媒组相比，GH给药组在不同时期的全身骨矿物质含量、骨矿物质密度（BMD）、骨面积和瘦体重显著增加，脂肪百分比降低;然而，变化幅度因给药期而异。例如，全身BMD的增加显著（P
We previously found that the magnitude of skeletal deficits caused by GH deficiency varied during different growth periods. To test the hypothesis that the sensitivity to GH is growth period dependent, we treated GH-deficient lit/lit mice with GH (4 mg/kg body weight.d) or vehicle during the prepubertal and pubertal (d 7-34), pubertal (d 23-34), postpubertal (d 42-55), and adult (d 204-217) periods and evaluated GH effects on the musculoskeletal system by dual energy x-ray absorptiometry (DEXA) and peripheral quantitative computed tomography. GH treatment during different periods significantly increased total body bone mineral content, bone mineral density (BMD), bone area, and lean body mass and decreased percentage of fat compared with vehicle; however, the magnitude of change varied markedly depending on the treatment period. For example, the increase in total body BMD was significantly (P