Nycthemeral differences in response to restraint stress in CD-1 and C57BL/6 mice
Nycthemeral differences in response to restraint stress in CD-1 and C57BL/6 mice
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DOI：
10.1016/j.physbeh.2003.09.010
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发表时间：
2004-01-01
影响因子：
2.9
通讯作者：
Nelson, RJ
中科院分区：
文献类型：
--
作者：
Hotchkiss, AK;Pyter, LM;Nelson, RJ
克制代表心理和身体上的压力。建立束缚应激小鼠模型的方法不同，其持续时间、束缚时间和所测小鼠的品系各不相同。这项研究的目标是：(1)确定压力反应最大化的最佳每日时间段；(2)描述小鼠品系对束缚反应的差异(如果有的话)。近交系CD-1和近交系C57BL/6小鼠在每日明暗周期的三个时间点被限制3h：(1)晚明相，(2)明暗过渡阶段，(3)中暗周期。另外的小鼠作为食物剥夺的对照组，或除采血外未被处理。两个品系的小鼠在束缚3天后体重均显著下降。无节制的缺乏食物的小鼠体重下降，特别是在过渡期缺乏食物的情况下。与对照组相比，束缚组小鼠的皮质酮水平升高。基础皮质酮和约束后皮质酮在不同菌株之间均无差异。在CD-1小鼠的过渡期和C57小鼠的过渡期和黑暗时期，由于缺乏食物，皮质酮都会升高。受抑的CD-1小鼠的皮质酮反应在暗期增强。这些结果表明，两个品系对束缚的生理反应是相似的。然而，皮质酮对限制和食物剥夺的反应在过渡和黑暗阶段最高。(C)2003 Elsevier Inc.保留所有权利。
Restraint represents psychological and physical stress. Methods used to model restraint stress in mice vary in duration, time of day during which restraint is applied, and the strain of mouse tested. The goals of this study were: (1) to identify the optimal daily time periods during which the stress response is maximized, and (2) to describe mouse strain differences, if any, in response to restraint. Groups of outbred CD-1 and inbred C57BL/6 mice were restrained for 3 h during three time points of the daily light-dark cycle: (1) the late light phase, (2) the transition between the light phase and the dark phase, and (3) the mid-dark phase. Additional mice served as control groups for food deprivation or were unhandled except for blood sampling. Mice of both strains lost significant body mass after 3 days of restraint. Unrestrained food-deprived mice lost body mass, particularly if food-deprived during transition periods. Corticosterone was elevated in restrained mice compared with control mice. Neither basal nor postrestraint corticosterone differed between strains. Corticosterone was elevated by food deprivation during transitional periods in CD-1 mice and during both transition and dark phases in C57 mice. Corticosterone response in restrained CD-1 mice was increased during the dark phase. These results suggest that the physiological response to restraint is similar in both strains. However, corticosterone responses to both restraint and food deprivation were highest during the transitional and dark phases. (C) 2003 Elsevier Inc. All rights reserved.