Role of estrogen deficiency in the formation and progression of cerebral aneurysms. Part I: experimental study of the effect of oophorectomy in rats
Role of estrogen deficiency in the formation and progression of cerebral aneurysms. Part I: experimental study of the effect of oophorectomy in rats
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DOI：
10.3171/jns.2005.103.6.1046
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发表时间：
2005-12-01
影响因子：
4.1
通讯作者：
Satoh, K
中科院分区：
文献类型：
--
作者：
Jamous, MA;Nagahiro, S;Satoh, K
物体。雌激素已被证明在血管生物学中起着核心作用。虽然它可能发挥有益的血管效应，但它在脑动脉瘤发病机制中的作用仍有待确定。为了进一步阐明激素的作用，作者研究了双侧卵巢切除对大鼠脑动脉瘤形成和发展的影响。将45只7周龄雌性SD大鼠分为三组。I组为正常大鼠(对照组)。组II和组III结扎右侧颈总动脉和双侧肾后动脉建立脑动脉瘤模型。1个月后，第11组大鼠行双侧卵巢切除术。实验开始三个月后，所有动物都被处死，制备脑血管腐蚀模型，并用扫描电子显微镜筛查脑动脉瘤。测定血浆雌二醇水平和明胶酶活力。除对照组外，其余大鼠均出现高血压。雌二醇水平II组显著低于其他组(P<；0.01)。脑动脉瘤发生率(60%)是脑动脉瘤组(20%)的3倍，脑动脉瘤平均直径(76+/-27微米，平均+/-标准差)大于三组(28+/-4.6微米)(p<；0.05)。对照组(第1组)无动脉瘤形成，三组间血浆明胶酶活性差异无统计学意义。大鼠脑动脉瘤模型重复性好。双侧卵巢切除增加了大鼠脑动脉瘤的易感性，提示激素在脑动脉瘤的发病机制中起一定作用。
Object. Estrogen has been shown to play a central role in vascular biology. Although it may exert beneficial vascular effects, its role in the pathogenesis of cerebral aneurysms remains to be determined. To elucidate the role of hormones further, the authors examined the effects of bilateral oophorectomy on the formation and progression of cerebral aneurysms in rats.Methods. Forty-five female, 7-week-old Sprague-Dawley rats were divided into three equal groups. Group I consisted of intact rats (controls). To induce cerebral aneurysms, the animals in Groups II and III were subjected to ligation of the right common carotid and bilateral posterior renal arteries. One month later, the rats in Group 11 underwent bilateral oophorectomy. Three months after the experiment began all animals were killed and cerebral vascular corrosion casts were prepared and screened for cerebral aneurysms by using a scanning electron microscope. Plasma was used to determine the level of estradiol and the gelatinase activity.Hypertension developed in all rats except those in the control group. The estradiol level was significantly lower in Group II than in the other groups (p < 0.01). The incidence of cerebral aneurysm formation in Group II (60%) was three times higher than that in Group III (20%), and the mean size of aneurysms in Group II (76 +/- 27 mu m, mean +/- standard deviation) was larger than that in Group III (28 +/- 4.6 mu M) (p < 0.05). No aneurysm developed in control animals (Group 1), and there was no significant difference in plasma gelatinase activity among the three groups.Conclusions. The cerebral aneurysm model was highly reproducible in rats. Bilateral oophorectomy increased the susceptibility of rats to aneurysm fori-nation, indicating that hormones play a role in the pathogenesis of cerebral aneurysms.