Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease
Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease
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DOI：
10.1128/jvi.00999-09
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发表时间：
2010-01-01
影响因子：
5.4
通讯作者：
Wong, Kum Thong
中科院分区：
文献类型：
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作者：
Ong, Kien Chai;Devi, Shamala;Wong, Kum Thong
肠道病毒71型(EV71)会导致儿童手足口病和神经系统并发症，目前还没有疫苗或治疗药物。在EV71脑脊髓炎小鼠模型上，对来自EV71临床分离株的甲醛灭活全病毒疫苗和一种小鼠适应病毒(MAV)进行了测试。在对出生1天和7日龄的小鼠只进行了两次免疫后，MAV疫苗保护了出生14天的小鼠免受疾病的侵袭。经病毒培养、逆转录聚合酶链式反应、免疫组织化学和原位杂交检测，免疫小鼠组织均未检出病毒。免疫的成年小鼠产生的针对B3、B4和C1至C5基因株的交叉中和EV71抗体能够被动保护14日龄小鼠免受疾病侵袭。
Enterovirus 71 (EV71) causes childhood hand, foot, and mouth disease and neurological complications, and no vaccines or therapeutic drugs are currently available. Formaldehyde-inactivated whole-virus vaccines derived from EV71 clinical isolates and a mouse-adapted virus (MAV) were tested in a mouse model of EV71 encephalomyelitis. After only two immunizations, given to mice at 1 and 7 days of age, the MAV vaccine protected mice at 14 days of age from disease. Tissues from immunized mice were negative for virus by viral culture, reverse transcriptase PCR, immunohistochemistry analysis, and in situ hybridization. Cross-neutralizing EV71 antibodies to strains with genotypes B3, B4, and C1 to C5 generated in immunized adult mice were able to passively protect 14-day-old mice from disease.