Human monoclonal antibody that recognizes the V3 region of human immunodeficiency virus gp120 and neutralizes the human T-lymphotropic virus type IIIMN strain.
Human monoclonal antibody that recognizes the V3 region of human immunodeficiency virus gp120 and neutralizes the human T-lymphotropic virus type IIIMN strain.
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人单克隆抗体，可识别人类免疫缺陷病毒 gp120 的 V3 区并中和人类嗜 T 淋巴细胞病毒 IIIMN 型毒株。
DOI：
10.1073/pnas.87.21.8597
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发表时间：
1990
影响因子：
11.1
通讯作者：
Robinson,JE
中科院分区：
文献类型：
--
作者：
ScottJr,CF;Silver,S;Profy,AT;Putney,SD;Langlois,A;Weinhold,K;Robinson,JE
我们描述了一种人类IgG1单克隆抗体（N701.9b），该抗体是由eb病毒转化来自人类免疫缺陷病毒血清阳性无症状供者的B细胞获得的。通过结合MN- gp120的PB-1片段和MN及相关病毒株V3区对应的合成肽，证明该抗体能够识别人类免疫缺陷病毒MN株和MN样病毒株gp120 V3区包含的主要中和结构域。单克隆抗体N701.9b鉴定的表位定位于MN菌株V3环的“尖端”和“右侧”，包含至少7个氨基酸（氨基酸316-322）的V3片段。此外，该抗体对MN病毒株表现出有效的类型特异性融合抑制活性，而对IIIB或RF病毒株则没有。该抗体也中和了4个具有mn样V3区序列的病毒分离株，但未能中和另外3个含有不相关V3区序列的病毒株。我们的研究结果证实，在人类自然感染人类免疫缺陷病毒期间，V3区刺激类型特异性中和抗体。讨论了该抗体的潜在临床应用。
We describe a human IgG1 monoclonal antibody (N701.9b) derived by Epstein-Barr virus transformation of B cells from a human immunodeficiency virus-seropositive asymptomatic donor. This antibody was shown to recognize the principal neutralizing domain contained within the V3 region of gp120 of the MN strain of human immunodeficiency virus and MN-like strains, as determined by binding to the PB-1 fragment of MN gp120 and to synthetic peptides corresponding to the V3 region of MN and related virus strains. The epitope identified by monoclonal antibody N701.9b was mapped to a segment of V3 containing at least 7 amino acids (amino acids 316-322), which is located in the "tip" and "right" side of the V3 loop of the MN strain. Furthermore, this antibody manifested potent type-specific fusion-inhibitory activity against the MN strain but not against the IIIB or RF virus strains. This antibody also neutralized four virus isolates that had MN-like V3 region sequences and failed to neutralize three other strains containing unrelated V3 region sequences. Our findings confirm that the V3 region stimulates type-specific neutralizing antibody during natural human immunodeficiency virus infection in humans. The potential clinical use of this antibody is discussed.