The extracellular domain of teneurin-4 promotes cell adhesion for oligodendrocyte differentiation
The extracellular domain of teneurin-4 promotes cell adhesion for oligodendrocyte differentiation
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DOI：
10.1016/j.bbrc.2019.12.002
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发表时间：
2020-02-26
影响因子：
3.1
通讯作者：
Akazawa, Chihiro
中科院分区：
文献类型：
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作者：
Hayashi, Chikako;Suzuki, Nobuharu;Akazawa, Chihiro
少突胶质细胞和神经元轴突之间的细胞粘附是髓鞘形成的关键步骤，髓鞘形成使得动作电位在中枢神经系统中快速传播。在这里，我们表明，跨膜蛋白teneurin-4在少突胶质细胞分化所需的细胞粘附中发挥作用。我们发现，teneurin-4与所有四个teneurin家族成员形成分子复合物，并促进细胞-细胞粘附。少突胶质细胞系细胞附着于所有端神经元的重组胞外结构域，并形成分支良好的细胞突起。在轴突模拟纳米纤维测定中，用重组teneurin-4胞外结构域包被的纳米纤维增加了少突胶质细胞的分化。我们的研究结果表明，teneurin-4结合所有teneurin通过其细胞外结构域，这有利于少突胶质细胞-轴突粘附，并促进少突胶质细胞的分化，通过其嗜同性相互作用。(C)2019爱思唯尔公司All rights reserved.
Cell adhesion between oligodendrocytes and neuronal axons is a critical step for myelination that enables the rapid propagation of action potential in the central nervous system. Here, we show that the transmembrane protein teneurin-4 plays a role in the cell adhesion required for the differentiation of oligodendrocytes. We found that teneurin-4 formed molecular complexes with all of the four teneurin family members and promoted cell-cell adhesion. Oligodendrocyte lineage cells attached to the recombinant extracellular domain of all the teneurins and formed well-branched cell processes. In an axon-mimicking nanofibers assay, nanofibers coated with the recombinant teneurin-4 extracellular domain increased the differentiation of oligodendrocytes. Our results show that teneurin-4 binds to all teneurins through their extracellular domain, which facilitates the oligodendrocyte-axon adhesion, and promotes oligodendrocyte differentiation via its homophilic interaction. (C) 2019 Elsevier Inc. All rights reserved.