Fabrication of Carbohydrate Surfaces by Using Nonderivatised Oligosaccharides, and their Application to Measuring the Assembly of Sugar–Protein Complexes

Fabrication of Carbohydrate Surfaces by Using Nonderivatised Oligosaccharides, and their Application to Measuring the Assembly of Sugar–Protein Complexes
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使用非衍生化低聚糖制造碳水化合物表面及其在测量糖-蛋白质复合物组装中的应用

DOI:
10.1002/cbic.200800696
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发表时间:
2009
期刊:
影响因子:
3.2
通讯作者:
D. Fernig
D. Fernig
中科院分区:
生物学3区
文献类型:
--
作者:
J. Popplewell;M. Swann;Yassir A. Ahmed;J. Turnbull;D. Fernig

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就这样往上走双偏振干涉测量法用于设计和表征表面,在该表面上固定碳水化合物的方向和密度适合研究它们与蛋白质的相互作用。乳铁蛋白显示出采用两种方向:“端上”或“侧上”,而对于 FGF-2,观察到单个单层蛋白质。新的表面可用于阐明蛋白质与碳水化合物的结合以及复合物的几何形状,这是一个经常引起争议的领域。
This way up. Dual polarisation interferometry was used to design and characterise a surface on which the orientation and density of immobilised carbohydrates was suitable for studying their interactions with proteins. Lactoferrin was shown to adopt two orientations: “end‐on” or “side‐on”, while for FGF‐2 a single monolayer of protein was observed. The new surface can be used to elucidate the binding of proteins to carbohydrates and the geometry of the complexes, a frequently controversial area.
DOI: 10.1006/jmbi.1997.1386
发表时间: 1997-11-28
影响因子: 5.6
作者:
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通讯作者: Baker, EN
DOI: 10.1073/pnas.88.8.3441
发表时间: 1991-04-01
影响因子: 11.1
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发表时间: 1995
期刊: Archives of biochemistry and biophysics.
影响因子: --
作者:
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通讯作者: Church,FC