Fabrication of Carbohydrate Surfaces by Using Nonderivatised Oligosaccharides, and their Application to Measuring the Assembly of Sugar–Protein Complexes
Fabrication of Carbohydrate Surfaces by Using Nonderivatised Oligosaccharides, and their Application to Measuring the Assembly of Sugar–Protein Complexes
复制标题
使用非衍生化低聚糖制造碳水化合物表面及其在测量糖-蛋白质复合物组装中的应用
DOI:
10.1002/cbic.200800696
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发表时间:
2009
期刊:
影响因子:
3.2
通讯作者:
D. Fernig
中科院分区:
文献类型:
--
作者:
J. Popplewell;M. Swann;Yassir A. Ahmed;J. Turnbull;D. Fernig
This way up. Dual polarisation interferometry was used to design and characterise a surface on which the orientation and density of immobilised carbohydrates was suitable for studying their interactions with proteins. Lactoferrin was shown to adopt two orientations: “end‐on” or “side‐on”, while for FGF‐2 a single monolayer of protein was observed. The new surface can be used to elucidate the binding of proteins to carbohydrates and the geometry of the complexes, a frequently controversial area.
影响因子:
5.6
作者:
Moore, SA;Anderson, BF;Baker, EN
通讯作者:
Baker, EN
DOI:
10.1073/pnas.88.8.3441
发表时间:
1991-04-01
影响因子:
11.1
作者:
ERIKSSON, AE;COUSENS, LS;MATTHEWS, BW
通讯作者:
MATTHEWS, BW
DOI:
10.1006/abbi.1995.1139
发表时间:
1995
期刊:
Archives of biochemistry and biophysics.
影响因子:
--
作者:
Wu,HF;Monroe,DM;Church,FC
通讯作者:
Church,FC