Isolation of 2-phenylethanol biosynthesis related genes and their relationship with 2-phenylethanol accumulation in Rosa rugosa
Isolation of 2-phenylethanol biosynthesis related genes and their relationship with 2-phenylethanol accumulation in Rosa rugosa
复制标题
玫瑰中2-苯乙醇生物合成相关基因的分离及其与2-苯乙醇积累的关系
DOI：
10.1007/s11738-015-1996-3
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Lixia Sheng
中科院分区：
文献类型：
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作者：
Liguo Feng;Meng Wang;Jia Wang;Shu Zang;Wei Xia;Lixia Sheng
玫瑰是中国的传统名花，不仅是一种园林植物，也是一种历史悠久、名贵的芳香植物。天然芳香物质2-苯乙醇是月季花和月季精油的主要不可缺少的成分。本研究以‘唐红’为试材，分离了2-苯乙醇生物合成相关基因RrAADC和RrPAR。测定了这两个基因在不同花发育阶段和花器官中的时空表达模式，确定了2-苯乙醇积累与RrAADC和RrPAR表达的关系。随着开花程度的增加，2-苯乙醇的含量逐渐增加，并在枯萎期达到峰值。随着花朵开放程度的增加，RrAADC的表达水平逐渐降低。同时，RrPAR的表达水平从现蕾期到半开口期逐渐升高，在全开口期迅速下降，到枯萎期又略有增加。花瓣中2-苯乙醇含量最高，雄蕊和雌蕊次之。然而，在完全开放的花的其他部分没有检测到这种化合物。RrAADC在雄蕊中的表达水平最高，在其他部位的表达水平相对较低。RrPAR在雄蕊和花萼中的表达水平最高，其次是雌蕊和花托，在花瓣和花梗中的表达水平最低。这些结果表明，RrAADC和RrPAR协同调节皱纹夜蛾2-苯乙醇的生物合成。
Rosa rugosa is a famous traditional flower in China used not only as a landscape plant but also as a time-honored and valuable aromatic plant. The natural aromatic substance 2-phenylethanol is the major indispensable ingredient of rose flower and rose essential oil. This study adopted R. rugosa ‘Tanghong’ as a test material to isolate 2-phenylethanol biosynthesis related genes RrAADC and RrPAR. The temporal and spatial expression patterns of the two genes in different flower developmental stages and floral organ parts were measured, and the relationship of 2-phenylethanol accumulation to RrAADC and RrPAR expression in R. rugosa was determined. The content of 2-phenylethanol in R. rugosa gradually increased with the degree of flower opening and peaked at the withering stage. The expression level of RrAADC gradually decreased with the degree of flower opening. Meanwhile, the expression level of RrPAR gradually increased from the budding stage to the half opening stage, rapidly decreased at the full opening stage, and slightly increased again toward the withering stage. The content of 2-phenylethanol was the highest in the petals, followed by that in the stamens and pistils. However, this compound was not detected in other parts of the fully opened flower. The expression level of RrAADC peaked in the stamens but was relatively low in the other parts. The expression level of RrPAR was the highest in the stamens and calyxes, followed by that in the pistils and receptacles, and the lowest in the petals and stalks. These results suggest that RrAADC and RrPAR coordinately regulate the biological synthesis of 2-phenylethanol in R. rugosa.