Sequence variation identified in the 18S rRNA gene of Theileria mutans and Theileria velifera from the African buffalo (Syncerus caffer)
Sequence variation identified in the 18S rRNA gene of Theileria mutans and Theileria velifera from the African buffalo (Syncerus caffer)
复制标题
DOI：
10.1016/j.vetpar.2012.08.005
复制
发表时间：
2013-01-16
影响因子：
2.6
通讯作者：
Oosthuizen, Marinda C.
中科院分区：
文献类型：
--
作者：
Chaisi, Mamohale E.;Collins, Nicola E.;Oosthuizen, Marinda C.
非洲布法罗（Synceruscaffer）是致病性和非致病性泰勒虫的天然储存宿主。这些通常在布法罗中以混合感染的形式自然发生。虽然良性和轻度致病性形式没有任何重大的经济意义，但它们的存在可能会使旨在对南非牛和布法罗中致病性小泰勒虫进行特异性诊断的诊断试验结果的解释复杂化。18 S rRNA基因已被用作实时定量PCR（qPCR）检测T. parva感染。然而，在非致病性泰勒虫属物种中该基因内的序列变异的程度。非洲布法罗的研究并不为人所知。本研究的目的是克隆变形泰勒虫、泰勒虫MSD株和泰勒虫T.并确定任何序列变异对T. velifera特异性检测的可能影响。使用18 S rRNA qPCR检测parva。使用反向线印迹（RLB）杂交测定来选择对几种不同泰勒虫属物种测试为阳性的样品，或仅与巴氏杆菌属/泰勒虫属特异性探针杂交而不与巴氏杆菌属或泰勒虫属物种特异性探针中的任一种杂交。对来自南非不同地区的13头布法罗和1头牛的14个样品的18 S rRNA基因进行了扩增、克隆和测序。在T.变形泰勒氏菌（Thileria sp.）velifera，其中T. mu tans变种。这种变异可能解释了RLB杂交试验不能检测T. mutans和T.在一些分析的样品中发现了velifera。(C)2012爱思唯尔有限公司版权所有。
The African buffalo (Syncerus caffer) is a natural reservoir host for both pathogenic and nonpathogenic Theileria species. These often occur naturally as mixed infections in buffalo. Although the benign and mildly pathogenic forms do not have any significant economic importance, their presence could complicate the interpretation of diagnostic test results aimed at the specific diagnosis of the pathogenic Theileria parva in cattle and buffalo in South Africa. The 18S rRNA gene has been used as the target in a quantitative real-time PCR (qPCR) assay for the detection of T. parva infections. However, the extent of sequence variation within this gene in the non-pathogenic Theileria spp. of the Africa buffalo is not well known. The aim of this study was, therefore, to characterise the full-length 185 rRNA genes of Theileria mutans, Theileria sp. (strain MSD) and T. velifera and to determine the possible influence of any sequence variation on the specific detection of T. parva using the 18S rRNA qPCR. The reverse line blot (RLB) hybridization assay was used to select samples which either tested positive for several different Theileria spp., or which hybridised only with the Babesia/Theileria genus-specific probe and not with any of the Babesia or Theileria species-specific probes. The full-length 18S rRNA genes from 14 samples, originating from 13 buffalo and one bovine from different localities in South Africa, were amplified, cloned and the resulting recombinants sequenced. Variations in the 18S rRNA gene sequences were identified in T. mutans, Theileria sp. (strain MSD) and T. velifera, with the greatest diversity observed amongst the T. mu tans variants. This variation possibly explained why the RLB hybridization assay failed to detect T. mutans and T. velifera in some of the analysed samples. (C) 2012 Elsevier B.V. All rights reserved.