Soluble group- and type-specific antigens from type III group B Streptococcus
Soluble group- and type-specific antigens from type III group B Streptococcus
复制标题
来自 III 型 B 组链球菌的可溶性群和类型特异性抗原
作者：
R. Carey;T. Eisenstein;G. Shockman;T. Greber;R. M. Swenson
在补充有酸水解酪蛋白的化学成分确定的肉汤中，菌株M732生长后，从培养基中分离出两种III型B群链球菌的可溶性多糖抗原。通过用二乙基氨基乙基-Sephacel的阴离子交换层析从培养上清液中分离型特异性抗原和群特异性抗原。分别与B组或III型抗血清具有血清学反应性的两个含碳水化合物的峰用线性NaCl梯度洗脱，并通过凝胶过滤进一步纯化。发现III型多糖含有葡萄糖、半乳糖、葡糖胺和唾液酸，而B组多糖含有半乳糖、葡糖胺和鼠李糖。对于III型多糖，唾液酸被证明是主要的免疫决定簇，而对于B组多糖，鼠李糖是免疫优势糖。III型和B类多糖均以高产率获得，而不采用苛刻的物理或化学处理，并且两者在免疫学上不同。经免疫电泳或反向免疫电泳，III型抗原与群特异性抗血清不反应，B群抗原与III型抗血清不反应。
Two soluble polysaccharide antigens of a type III group B Streptoccus were isolated from the culture medium after growth of strain M732 in a chemically defined broth supplemented with acid-hydrolyzed casein. The type- and group- specific antigens were isolated from the culture supernatant by anion-exchange chromatography with diethylaminoethyl-Sephacel. Two carbohydrate-containing peaks, which had serological reactivity with group B or type III antiserum, respectively, were eluted with a linear NaCl gradient and further purified by gel filtration. The type III polysaccharide was found to contain glucose, galactose, glucosamine, and sialic acid, whereas the group B polysaccharide contained galactose, glucosamine, and rhamnose. For the type III polysaccharide, sialic acid was shown to be the major immunodeterminant, and for the group B polysaccharide, rhamnose was the immunodominant sugar. Both the type III and group B polysaccharides were obtained in high yields without employing harsh physical or chemical treatment and both were immunologically distinct. By immunoelectrophoresis or counterimmunoelectrophoresis, type III antigen failed to react with group-specific antiserum and the group B antigen failed to react with type III antiserum.