Establishment and comparison of Actinobacillus pleuropneumoniae experimental infection model in mice and piglets
Establishment and comparison of Actinobacillus pleuropneumoniae experimental infection model in mice and piglets
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DOI：
10.1016/j.micpath.2019.01.028
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发表时间：
2019-03-01
影响因子：
3.8
通讯作者：
Lei, Lian-cheng
中科院分区：
文献类型：
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作者：
Bao, Chun-tong;Xiao, Jia-meng;Lei, Lian-cheng
胸膜肺炎放线杆菌（APP）引起猪胸膜肺炎，这是一种在世界范围内养猪业造成重大损失的疾病。本研究以昆明种小鼠（KM）和中国肿瘤研究所小鼠（ICR）作为APP研究的替代模型。鼻内感染血清5型参考菌株L20后，ICR小鼠的肺损伤和临床体征评分均低于KM小鼠。而ICR小鼠在体重减轻、血液和支气管肺泡灌洗液（BALF）中免疫细胞（如中性粒细胞和淋巴细胞）和细胞因子（如IL-6、IL-1 β和TNF-α）的数量方面表现出更明显的变化。在ICR小鼠中观察到的免疫学变化与在感染L20的仔猪中观察到的免疫学变化非常相似。虽然ICR和KM小鼠都对APP敏感并诱导病理损伤，但我们认为ICR和KM小鼠分别更适合于免疫学和发病机制研究。本研究为确定小鼠可替代猪作为APP感染模型奠定了理论基础，对APP感染的实验室研究具有重要意义。
Actinobacillus pleuropneumoniae (APP) causes porcine pleuropneumonia, a disease responsible for substantial losses in the worldwide pig industry. In this study, outbred Kunming (KM) and Institute of Cancer Research (ICR) mice were evaluated as alternative mice models for APP research. After intranasal infection of serotype 5 reference strain L20, there was less lung damage and a lower clinical sign score in ICR compared to KM mice. However, ICR mice showed more obvious changes in body weight loss, the amount of immune cells (such as neutrophils and lymphocytes) and cytokines (such as IL-6, IL-1 beta and TNF-alpha) in blood and bronchoalveolar lavage fluid (BALF). The immunological changes observed in ICR mice closely mimicked those found in piglets infected with L20. While both ICR and KM mice are susceptible to APP and induce pathological lesions, we suggest that ICR and KM mice are more suitable for immunological and pathogenesis studies, respectively. The research lays the theoretical basis for determine that mice could replace pigs as the APP infection model and it is of significance for the study of APP infection in the laboratory.