Genetic variability and evolution of rice stripe virus
Genetic variability and evolution of rice stripe virus
复制标题
水稻条纹病毒的遗传变异和进化
DOI：
10.1631/jzus.b1300014
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发表时间：
2013-10-01
影响因子：
5.1
通讯作者：
Wu, Jian-xiang
中科院分区：
文献类型：
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作者：
Huang, Ling-zhe;Rao, Li-xia;Wu, Jian-xiang
水稻条纹病毒（Rice Stripe Virus，RSV）是细病毒属（Tenuivirus）的模式成员。已知RSV具有四个分段的单链RNA分子，并在中国、日本和韩国的稻田中引起水稻条纹病。根据GenBank中已测定的6个RSV分离株（来自中国云南、江苏、浙江和辽宁省）和27个其他RSV分离株（来自中国云南、江苏、安徽、河南和山东省以及日本和韩国）的全基因组序列，我们提供了RSV野外分离株的基因分型谱，并描述了RSV的群体结构。除CX株外，所有RSV分离株可分为2个亚型，其中云南省6株，中国、日本和韩国的26株，分别称为II亚型和I亚型。亚型之间的氨基酸距离范围为0.053至0.085。云南省RSV分离株与中国其他地区、日本和韩国的RSV分离株在遗传上存在差异，并显示出罕见的基因流动。从中国、日本和韩国其他地区收集的RSV群体仅由I亚型组成，并且显示出非常低的遗传多样性。我们推测，CX分离株可能是两个亚型分离株重组的结果。在CX株的RNA4中检测到两个潜在的重组事件。
Rice stripe virus (RSV) is the type member of the genus Tenuivirus. RSV is known to have four segmented, single-stranded RNA molecules and causes rice stripe disease in the rice fields of China, Japan, and Korea. Based on the complete genomic sequences of the determined 6 RSV isolates (from Yunnan, Jiangsu, Zhejiang, and Liaoning Provinces, China) and 27 other RSV isolates (from Yunnan, Jiangsu, Anhui, Henan, and Shandong Provinces of China, also Japan and Korea) downloaded from GenBank, we provided a genotyping profile of RSV field isolates and described the population structure of RSV. All RSV isolates, except isolate CX, could be divided into two subtypes, one including 6 isolates from Yunnan Province, and the other including 26 isolates from different parts of China, Japan, and Korea, which were referred to as subtype II and subtype I, respectively. The amino acid distances between subtypes range from 0.053 to 0.085. RSV isolates in Yunnan Province were genetically differentiated from other parts of China, Japan, and Korea and showed infrequent gene flow. The RSV populations collected from other parts of China, Japan, and Korea were only composed of subtype I and showed very low genetic diversity. We speculated that isolate CX may be the result of recombination of isolates from two subtypes. Two potential recombination events were detected in RNA4 of isolate CX.