Preparation of an iminodiacetic acid-modified capillary and its performance in capillary liquid chromatography and immobilized metal chelate affinity capillary electrophoresis.
Preparation of an iminodiacetic acid-modified capillary and its performance in capillary liquid chromatography and immobilized metal chelate affinity capillary electrophoresis.
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DOI：
10.1016/j.chroma.2004.03.063
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发表时间：
2004-06
期刊：
影响因子：
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通讯作者：
K. Tsukagoshi;Yasushi Shimadzu;Tatsuhiko Yamane;R. Nakajima
中科院分区：
文献类型：
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作者：
K. Tsukagoshi;Yasushi Shimadzu;Tatsuhiko Yamane;R. Nakajima
通过N-（乙烯基苄基亚氨基）二乙酸的聚合制备了亚氨基二乙酸（IDA）修饰和Cu（II）-IDA修饰的毛细管。在毛细管液相色谱（LC）和固定化金属螯合亲和毛细管电泳（IMACE）中考察了这些毛细管的基本性能。铜（II），钴（II），和血红素检测在不同的保留时间通过毛细管LC与化学发光检测器，在此期间，使用IDA-修饰的毛细管。保留时间的差异归因于金属离子或络合物与毛细管内壁上IDA部分之间的相互作用的差异。此外，采用Cu（II）-IDA修饰毛细管柱，用IMACE分离检测人血清白蛋白（HSA）和人血清γ-球蛋白（HγG）。通过蛋白质与毛细管内壁Cu（II）螯合基团的相互作用实现了HSA和HγG的分离。
We prepared iminodiacetic acid (IDA)-modified and Cu(II)–IDA-modified capillaries through polymerization of N-(vinylbenzylimino)diacetic acid. The fundamental performance of these capillaries was examined in capillary liquid chromatography (LC) and immobilized metal chelate affinity capillary electrophoresis (IMACE). Copper(II), cobalt(II), and hematin were detected at different retention times by means of capillary LC with a chemiluminescence detector, during which the IDA-modified capillary was used. The difference in the retention times was attributed to the difference in the interaction between metal ions or complex and IDA moieties on the inner wall of the capillary. In addition, human serum albumin (HSA) and human serum γ-globulin (HγG) were separated and detected using IMACE with an absorption detector, during which the Cu(II)–IDA-modified capillary was used. The separation of HSA and HγG was achieved through the interaction between proteins and Cu(II) chelate moieties on the inner wall of this capillary.