Targeted Metabolomics Identifies Differential Serum and Liver Amino Acids Biomarkers in Rats with Alcoholic Liver Disease.
Targeted Metabolomics Identifies Differential Serum and Liver Amino Acids Biomarkers in Rats with Alcoholic Liver Disease.
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DOI：
10.3177/jnsv.66.536
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发表时间：
2020-12
影响因子：
1.6
通讯作者：
Chenze Shi;Lei Wang;K. Zhou;Mingmei Shao;Yifei Lu;Tao Wu
中科院分区：
文献类型：
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作者：
Chenze Shi;Lei Wang;K. Zhou;Mingmei Shao;Yifei Lu;Tao Wu
探讨ALD患者血清及肝脏氨基酸水平的变化，为ALD的防治提供新的依据和方法。将20只无特定病原体SD雄性大鼠分为两组，对照组10只，模型组10只。测定血清生化指标，包括丙氨酸转氨酶、天冬氨酸转氨酶、层粘连蛋白和透明质酸酶。对肝组织进行组织学分析。采用基于超高效液相色谱-串联四极杆质谱（UPLC-TQMS）的靶向代谢组学方法定量测定血清和肝脏氨基酸水平。与正常组比较，ALD大鼠血清β-丙氨酸、丙氨酸、丝氨酸、鸟氨酸、酪氨酸水平和酪氨酸比值明显升高，精氨酸水平、BTR比值和Fischer比值明显降低。与正常组相比，ALD大鼠肝脏组织中肌氨酸、β-丙氨酸、丝氨酸、脯氨酸、缬氨酸、苏氨酸、鸟氨酸、赖氨酸、组氨酸、酪氨酸、对称二甲基精氨酸、蛋氨酸、异亮氨酸和蛋氨酸-亚砜含量明显降低，半胱氨酸和腐胺含量明显升高。血清和肝脏氨基酸在ALD大鼠中表现出显著的变化，可以被认为是ALD潜在的特异性诊断生物标志物。
To investigate changes in serum and hepatic levels of amino acids in ALD and to provide novel evidence and approaches for the prevention and treatment of ALD. Twenty specific pathogen-free SD male rats were devided into two groups, ten for the control group, and ten for the model group. Serum biochemical markers, including alanine aminotransferase, aspartate aminotransferase, laminin and hyaluronidase were measured. Histological analysis of liver tissues was performed. Serum and liver amino acids levels were quantitatively determined by ultra-high-performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC-TQMS)-based targeted metabolomics. Compared with the normal group, ALD rats showed an obvious increase in the levels of β-alanine, alanine, serine, ornithine, tyrosine and the tyrosine ratio, while there was a decrease in arginine levels, the BTR ratio and Fischer's ratio in serum. Additionally, ALD rats exhibited a significant increase in the levels of cysteine and putrescine, while there was a decrease in sarcosine, β-alanine, serine, proline, valine, threonine, ornithine, lysine, histidine, tyrosine, symmetric dimethylarginine, methionine, isoleucine and methionine-sulfoxide levels in liver tissues compared with the normal group. The serum and liver amino acids showed significant changes in ALD rats and can be considered as potential specific diagnostic biomarkers for ALD.