A transgenic mouse model for studying the clearance of blood-borne pathogens via human complement receptor 1 (CR1).
A transgenic mouse model for studying the clearance of blood-borne pathogens via human complement receptor 1 (CR1).
复制标题
用于研究通过人补体受体 1 (CR1) 清除血源性病原体的转基因小鼠模型。
DOI：
10.1111/j.1365-2249.2005.02764.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Finberg,RW
中科院分区：
文献类型：
--
作者：
Repik,A;Pincus,SE;Ghiran,I;Nicholson-Weller,A;Asher,DR;Cerny,AM;Casey,LS;Jones,SM;Jones,SN;Mohamed,N;Klickstein,LB;Spitalny,G;Finberg,RW
Complement receptor 1 (CR1) on the surface of human erythrocytes facilitates intravascular clearance of complement-opsonized pathogens. The need for complement activation can be circumvented by directly coupling the organism to CR1 using a bispecific monoclonal antibody heteropolymer (HP). Lack of a functional homologue to CR1 on mouse erythrocytes has made it difficult to study HP-dependent clearance of pathogens in small animals. We have developed a transgenic mouse that expresses human CR1 on erythrocytes. CR1 antigen is of appropriate size and in a clustered distribution as confirmed by immunoblotting and fluorescence microscopy, respectively. HP that immobilized bacteriophage ΦX174 prototype pathogen to erythrocyte CR1 of the transgenic mice increased the rate of clearance of the virus compared with HP that bound bacteriophage, but not CR1. This transgenic mouse model will allow evaluation of different HPs for theirin vivoefficacy and potential as human therapeutics.
