Study of the adsorptive behavior of water-soluble dye molecules (rhodamine 6G) at the air-water interface using confocal fluorescence microscope.
Study of the adsorptive behavior of water-soluble dye molecules (rhodamine 6G) at the air-water interface using confocal fluorescence microscope.
复制标题
DOI：
10.1016/s1386-1425(00)00389-9
复制
发表时间：
2001-02
期刊：
影响因子：
--
通讯作者：
X. Y. Zheng;A. Harata;T. Ogawa
中科院分区：
文献类型：
--
作者：
X. Y. Zheng;A. Harata;T. Ogawa
应用共聚焦荧光显微镜直接研究了水溶性发色团罗丹明6 G（R6 G）在其极低浓度区域（低于10− 10 M）在空气-水界面上吸附分子的特征行为。仅从表面观察到显著的光子爆发，并且发现它们的宽度、高度和频率取决于体积浓度，这表明R6 G分子在空气-水界面处的不均匀分布。吸附态分子的这种性质不同于体相分子。研究了离子强度对界面光子爆发的影响。结果发现，向R6 G溶液中加入NaCl导致荧光信号的降低。聚集体的大小和吸附分子的荧光量子产率的变化被认为是负责这个实验结果。
A confocal fluorescence microscope was applied to directly study the characteristic behaviors of adsorbed molecules at the air–water interface for a water-soluble chromophore, rhodamine 6G (R6G), in its extremely low-concentration region (below 10−10M). Significant photon bursts were observed only from the surface, and their width, height, and frequency were found to depend on the bulk concentration, suggesting the inhomogeneous distribution of R6G molecules at the air–water interface. This property of the adsorbed molecules is different from that of the bulk one. The influence of the ionic strength on photon bursts from the interface was investigated. It was found that the addition of NaCl to the R6G solution caused a decrease of the fluorescence signal. A change in the size of the aggregate and in the fluorescence quantum yield of the adsorbed molecules was suggested to be responsible for this experimental result.