Application of a novel open-source program for measuring the effects of toxicants on the swimming behavior of large groups of unmarked fish
Application of a novel open-source program for measuring the effects of toxicants on the swimming behavior of large groups of unmarked fish
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DOI：
10.1016/j.chemosphere.2011.11.011
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发表时间：
2012-03-01
期刊：
影响因子：
8.8
通讯作者：
Barry, Michael J.
中科院分区：
文献类型：
--
作者：
Barry, Michael J.
本研究的目的是展示一种新的方法来测量有毒物质对无标记鱼类群体行为的影响。Ctrax是一个开源的基于python的机器视觉程序，最初是为了追踪果蝇而设计的。在一个小的竞技场中测量了鱼类麻醉剂硫酸喹那丁对10只无标记阿拉伯鳉鱼（Aphanias dispar）群行为的影响。Ctrax能够在1分钟的记录周期内识别和跟踪单个鱼的X-Y坐标。利用专门编写的Matlab （The Mathworks(TM)）工具箱对数据进行分析，计算硫酸喹那丁对绝对游泳速度、正向游泳速度、方向变化率和离壁距离的影响。此外，还测定了硫酸喹那丁对路径扭曲的影响。在追踪鱼类的二维坐标方面，Ctrax比其他现有技术有显著的优势。特别是该软件是开源的，因此是免费的，它是准确的，只需要简单的容易获得的设备，能够跟踪潜在的大群鱼（可能50条），同时保持它们的个体身份。(C) 2011 Elsevier Ltd.版权所有。
The aim of this study was to demonstrate a novel method for measuring the effects of toxicants on the behavior of groups of unmarked fish. Ctrax is an open source Python-based machine vision program that was originally designed to track fruit flies (Drosophila). The effects of the fish anaesthetic quinaldine sulfate on the behavior of groups of 10 unmarked Arabian killifish (Aphanias dispar) in a small arena were measured. Ctrax was able to identify and track the X-Y coordinates of the individual fish over 1 min recording periods. The data was analyzed using specifically written Matlab (The Mathworks(TM)) toolboxes and the effects of quinaldine sulfate on absolute swimming velocity, forward swimming velocity, rate of change in orientation and distance to wall were calculated. Additionally the effects of quinaldine sulfate on path tortuosity were also measured. Ctrax has significant benefits over other available technologies for tracking the 2-dimensional coordinates of fish. In particular the software is open source and thus freely available, it is accurate, requires only simple easily available equipment and able to track potentially large groups of fish (possibly >50) while maintaining their individual identities. (C) 2011 Elsevier Ltd. All rights reserved.