Comparative Genomics of Atypical Enteropathogenic Escherichia coli from Kittens and Children Identifies Bacterial Factors Associated with Virulence in Kittens.
Comparative Genomics of Atypical Enteropathogenic Escherichia coli from Kittens and Children Identifies Bacterial Factors Associated with Virulence in Kittens.
复制标题
小猫和儿童非典型肠病性大肠杆菌的比较基因组学鉴定了与小猫毒力相关的细菌因素。
DOI：
10.1128/iai.00619-20
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发表时间：
2021
影响因子：
3.1
通讯作者：
Gookin,JodyL
中科院分区：
文献类型：
--
作者：
Watson,VictoriaE;Hazen,TracyH;Rasko,DavidA;Jacob,MeganE;Elfenbein,JohannaR;Stauffer,StephenH;Gookin,JodyL
典型的致肠性大肠埃希菌(TEPEC)是全球儿童腹泻和相关死亡的主要原因。非典型EPEC(AEPEC)缺乏编码束形成菌毛的质粒，被认为毒力较低，但毒力的分子机制尚不清楚。我们最近发现小猫是EPEC的宿主，肠道上皮细胞定植与腹泻疾病和死亡有关。本研究的目的是(I)确定猫aEPEC与人aEPEC分离株之间的基因组相似性，(Ii)鉴定与猫aEPEC毒力相关的基因或表型性状。我们没有观察到猫和人的aEPEC在核心基因组含量或基因簇序列同源性上的差异，也没有观察到来自非临床定植(NC)和致死性感染(LI)的aEPEC分离株的基因组内容有差别。培养细胞中黏附模式和聚集肌动蛋白的能力的变化反映了人类aEPEC的描述。从携带LI的幼猫体内分离的aEPEC比从携带NC的幼猫体内分离的aEPEC的运动能力更强。小猫可以作为aEPEC的储存库，与人类aEPEC分离株没有区别，并可能为aEPEC发病机制的研究提供必要的比较动物模型。运动性可能是导致幼猫急性早幼粒细胞白血病发生的重要因素。
Typical enteropathogenic Escherichia coli (tEPEC) is a leading cause of diarrhea and associated death in children worldwide. Atypical EPEC (aEPEC) lacks the plasmid encoding bundle-forming pili and is considered less virulent, but the molecular mechanism of virulence is poorly understood. We recently identified kittens as a host for aEPEC where intestinal epithelial colonization was associated with diarrheal disease and death. The purposes of this study were to (i) determine the genomic similarity between kitten aEPEC and human aEPEC isolates and (ii) identify genotypic or phenotypic traits associated with virulence in kitten aEPEC. We observed no differences between kitten and human aEPEC in core genome content or gene cluster sequence identities, and no distinguishing genomic content was observed between aEPEC isolates from kittens with nonclinical colonization (NC) versus those with lethal infection (LI). Variation in adherence patterns and ability to aggregate actin in cultured cells mirrored descriptions of human aEPEC. The aEPEC isolated from kittens with LI were significantly more motile than isolates from kittens with NC. Kittens may serve as a reservoir for aEPEC that is indistinguishable from human aEPEC isolates and may provide a needed comparative animal model for the study of aEPEC pathogenesis. Motility seems to be an important factor in pathogenesis of LI associated with aEPEC in kittens.