Laser direct-write patterned paper-based devices for detection of bacterial pathogens (Conference Presentation)
Laser direct-write patterned paper-based devices for detection of bacterial pathogens (Conference Presentation)
复制标题
用于检测细菌病原体的激光直写图案纸基设备（会议演示）
DOI：
10.1117/12.2506790
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
He P
中科院分区：
文献类型：
--
作者：
He P
抗菌素耐药性（AMR）已被世界卫生组织确定为全球性威胁，目前在欧洲和美国每年至少有5万人死亡，世界其他地区还有数十万人死亡。目前的常规经验性抗生素治疗方案涉及基于实验室的细菌培养测试，通常需要长达2-3天，是AMR全球流行的主要原因。因此，迫切需要低成本但可靠的护理点诊断解决方案，以快速和早期筛查感染，特别是在缺乏基础设施和训练有素人员的发展中国家。我们研究的目的是制造一种新型的纸基细菌培养装置，即使是不熟练的用户在低资源环境中也可以用于感染测试。在这里，我们提出了我们的初步结果，涉及使用我们独特的激光图案纸基设备检测和敏感性测试的大肠杆菌/Psealgia，泌尿道和上呼吸道感染的主要原因之一。这些纸器件是通过激光直写程序，使用c.w. 405 nm的激光以固化光聚合物并在纸基底内产生延伸穿过纸厚度的疏水壁，从而在流体装置内限定单独的测试区。我们的激光图案纸装置具有多个用琼脂浸渍的测试区（含有不同剂量的不同抗生素），仅允许选择性生长/抑制，从而通过简单的视觉可观察的颜色变化检测/鉴定大肠杆菌/假单胞菌。
Antimicrobial resistance (AMR) has been identified by the World Health Organisation as a global threat that currently claims at least 50,000 lives each year across Europe and the US, with many hundreds of thousands more dying in other areas of the world. The current routine empirical antibiotic therapy protocols that involve laboratory-based bacterial culture testing normally takes up to 2-3 days and are a primary contributor to the global prevalence of AMR. There is therefore an urgent need for low-cost but reliable point-of-care diagnostic solutions for rapid and early screening of infections especially in developing countries that have a lack of both infrastructure and trained personnel. The objective of our research is to fabricate a novel paper-based bacterial culture device that can be used for infection testing even by unskilled users in low resource settings. Here, we present our preliminary results relating to use of our unique laser-patterned paper-based devices for detection and susceptibility testing of E.Coli/Psedomonas, one of the leading causes of urinary tract and upper respiratory tract infections. These paper devices were fabricated via a laser direct-write procedure that uses c.w. laser light at 405nm to cure a photopolymer and produce within paper substrates hydrophobic walls that extend through the thickness of the paper thereby defining separate test zones within the fluidic device. Our laser-patterned paper device has multiple test zones impregnated with agar (containing different antibiotics in various doses) allowing only the selective growth/inhibition and thus detection/susceptibility-testing of E.coli/Pseudomonas via a simple visually observable colour change.