Hydroxyapatite particles as a controlled release carrier of protein
Hydroxyapatite particles as a controlled release carrier of protein
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DOI：
10.1016/j.biomaterials.2003.10.081
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Takahashi, J
中科院分区：
文献类型：
--
作者：
Matsumoto, T;Okazaki, M;Takahashi, J
本研究探讨了使用羟基磷灰石(HAP)颗粒作为蛋白质控释载体的可能性。为了实现蛋白质从羟基磷灰石颗粒中的有效释放，有必要调节蛋白质在羟基磷灰石上的结合量和羟基磷灰石的吸收。在湿法条件下，在不同温度(40℃、60℃、80℃)下合成了HAP粒子，并对合成的HAP粒子的物化性能进行了测试。低温合成的HAP颗粒结晶度低、溶解度高、比表面积大。骨形态发生蛋白、碱性成纤维细胞生长因子和转化生长因子-β等是骨再生有用的生长因子，因此以细胞色素c(pI=10.2)为模型蛋白，考察了蛋白质在羟基磷灰石颗粒上的吸附性能。蛋白质在HAP颗粒上的吸附随其比表面积的不同而不同，蛋白质在HAP颗粒上的吸附曲线符合典型的朗缪尔曲线。这些结果表明，蛋白质在HAP颗粒上的吸附量可以通过HAP的合成温度和蛋白质溶液的浓度来调节。在不同pH溶液(pH 4.0和7.0)中，研究了吸附蛋白质的HAP-PRO颗粒中蛋白质的释放动力学。HAP-PRO在pH 4.0溶液中的释放量随时间的增加而逐渐增加，而在pH 7.0溶液中的释放量明显小于HAP-PRO。此外，不同温度下合成的HAP-PRO的蛋白质释放速率不同，说明HAP-PRO的蛋白质释放依赖于HAP的吸收。这些结果表明，在不同温度下合成的羟基磷灰石颗粒可以作为蛋白质的控释载体。(C)2003爱思唯尔有限公司。保留所有权利。
This study examines the possibility of using hydroxyapatite (HAp) particles as a controlled release carrier of protein. In order to achieve effective protein release from HAp particles, it is necessary to regulate the conjugated amount of protein on HAp and the resorption of HAp. HAp particles were synthesized at different temperatures (40 degreesC, 60 degreesC, 80 degreesC) in wet condition and the physicochemical properties of synthesized HAp particles were examined. HAp particles synthesized at low temperatures showed low crystallinity, high solubility and large specific surface area. The useful growth factors for bone regeneration, such as BMP, bFGF and TGF-beta, are basic proteins, so cytochrome c (pI = 10.2) was used as a model protein and the adsorptive property of protein on HAp particles was investigated. The protein adsorption on HAp particles changed depending on its specific surface area and the chart of protein adsorption on HAp particles showed a typical Langmuir curve. These findings suggest that the adsorbed amount of protein on HAp particles could be regulated by HAp synthesizing temperature and the concentrations of protein solution. The release kinetics of protein from the HAp particles that adsorbed the protein (HAp-pro) was also evaluated in different pH solutions (pH 4.0 and 7.0). The released protein gradually increased time dependently when HAp-pro were immersed in pH 4.0 solution, but the released protein was significantly smaller when HAp-pro were immersed in pH 7.0 solution. Moreover, the release rate of protein from HAp-pro differed in each HAp that was synthesized at different temperatures, suggesting that the release of protein from HAp-pro depended on HAp resorption. These results suggest that HAp particles synthesized at different temperature are useful as a controlled release carrier of protein. (C) 2003 Elsevier Ltd. All rights reserved.