CD25+ CD4+ regulatory T cells in patients with Kawasaki disease
CD25+ CD4+ regulatory T cells in patients with Kawasaki disease
复制标题
DOI：
10.1016/j.jpeds.2004.05.048
复制
发表时间：
2004-09-01
影响因子：
5.1
通讯作者：
Hara, T
中科院分区：
文献类型：
--
作者：
Furuno, K;Yuge, T;Hara, T
目的探讨CD 25（+）CD 4（+）调节性T细胞群与川崎（KD）发病的关系。（中位年龄，30个月; 27名女性和27名男性患者）符合KD标准。配对的对照组包括17名活动性感染患者和24名健康儿童。We.检测CD 25（+）CD 4（+）细胞及外周血单个核细胞和纯化CD 4（+）T细胞中Foxp 3、细胞毒性T淋巴细胞相关抗原4（CTLA 4）、糖皮质激素诱导的肿瘤坏死因子受体（GITR）和转化生长因子β（TGF β）mRNA的表达（中位数，占总淋巴细胞的2.35%）显著低于健康对照组（中位数，3.14%）和有疾病的对照组（中位数，3.15%）。静脉注射丙种球蛋白治疗后，比例恢复到正常水平（中位数，3.86%）。结论KD患儿急性期CD 25（+）CD 4（+）调节性T细胞的减少可能与KD的发生发展有关。
Objective To investigate whether the CD25(+)CD4(+) regulatory T-cell population, which plays important roles not only in maintaining immunologic self-tolerance but also in controlling the magnitude and character of antimicrobial immune responses, is related to the pathophysiology of Kawasaki disease (KD).Study design The patient group consisted of 54 patients (median age, 30 months; 27 female and 27 male patients) fulfilling the criteria for KD. Age-matched control subjects included 17 patients with active infections and 24 healthy children. We. analyzed CD25(+)CD4(+) cells and the mRNA expression of Foxp3, cytotoxic T lymphocyte-associated antigen 4 (CTLA4), glucocorticoid-induced tumor necrosis factor receptor (GITR), and transforming growth factor beta in peripheral blood mononuclear cells and purified CD4(+) T cells.Results The proportions of CD25(+)CD4(+) cells inpatients with acute-phase KD (median, 2.35% of total lymphocytes) were significantly lower than those in healthy control subjects (median, 3.14%) and control subjects with disease (median, 3.15%). The proportions returned to the normal level after intravenous gammaglobulin treatment (median, 3.86%). The mRNA expression of Foxp3, CTLA4, and GITR showed similar tendencies.Conclusions The decrease of CD25(+)CD4(+) regulatory T cells in the acute phase might have a role in the development of KD.