Crocin "saffron" protects against beryllium chloride toxicity in rats through diminution of oxidative stress and enhancing gene expression of antioxidant enzymes
Crocin "saffron" protects against beryllium chloride toxicity in rats through diminution of oxidative stress and enhancing gene expression of antioxidant enzymes
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DOI：
10.1016/j.ecoenv.2012.06.003
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发表时间：
2012-09-01
影响因子：
6.8
通讯作者：
Alghaithy, Abdulaziz A. A.
中科院分区：
文献类型：
--
作者：
El-Beshbishy, Hesham A.;Hassan, Memy H.;Alghaithy, Abdulaziz A. A.
氯化铍（BeCl2）是一种剧毒物质，吸收后会在不同组织中积聚。本研究的目的是研究藏红花素对大鼠 BeCl2 中毒的保护作用。本研究使用雄性 Wistar 大鼠并分为四组 (n=8)。 I组作为正常对照大鼠。第 II 组口服 BeCl2 86 mg/kg b.w. 治疗。连续五天。该剂量相当于实验LD50。第III组用藏红花素200mg/kg体重腹膜内治疗。连续七天。第 IV 组在 BeCl2 给药前连续 7 天接受藏红花素。获取血液样本以及肝脏和脑匀浆用于血液学、生化和RT-PCR检查。 BeCl2处理后的大鼠的血细胞比容值、红细胞计数和血红蛋白浓度显着降低。与对照组相比，BeCl2暴露组大鼠肝脏和脑部丙二醛水平和蛋白质羰基含量显着增加，而给予藏红花素后这些值显着下降。与对照组相比，大鼠肝脏和大脑中的乳酸脱氢酶水平显着升高，并且与过氧化氢酶和超氧化物歧化酶活性显着降低相关。 BeCl2处理后大鼠肝脏还原型谷胱甘肽含量显着降低。与正常大鼠相比，BeCl2中毒大鼠过氧化氢酶和超氧化物歧化酶基因的mRNA表达显着下降。在摄入 BeCl2 之前进行藏红花素处理，导致过氧化氢酶和超氧化物歧化酶基因的 mRNA 表达显着增加，接近正常水平。血液学和生化参数恢复到接近正常水平。我们的结果表明，BeCl2 诱导细胞脂质和蛋白质的氧化；服用藏红花素可以减少 BeCl2 诱导的氧化应激，同时启动抗氧化基因的 mRNA 表达。 (c) 2012 Elsevier Inc. 保留所有权利。
Beryllium chloride (BeCl2) is a highly toxic substance that accumulates in different tissues after absorption. The purpose of this study was to investigate protective role of crocin against BeCl2-intoxication in rats. Male Wistar rats were used in this study and categorised into four groups (n=8). Group I served as normal control rats. Group II treated orally with BeCl2 86 mg/kg b.w. for five consecutive days. This dose was equivalent to experimental LD50. Group III treated intraperitoneally with crocin 200 mg/kg b.w. for seven consecutive days. Group IV received crocin for seven consecutive days before BeCl2 administration. Blood samples and liver and brain homogenates were obtained for haematological, biochemical and RT-PCR examinations. The haematocrit value, RBCs count and haemoglobin concentration were significantly decreased in BeCl2-treated rats. A significant increase was observed in rat liver and brain malondialdehyde level and protein carbonyls content in BeCl2 exposed group compared to the control group, and these values were significantly declined upon administration of crocin. Lactate dehydrogenase levels in rat liver and brain significantly increased compared to the control group and was associated with significant decrease in catalase and superoxide dismutase activities. Reduced glutathione hepatic contents of BeCl2-treated rats were significantly decreased. There was significant decline in mRNA expression of catalase and superoxide dismutase genes in BeCl2-intoxicated rats compared to the normal rats. Crocin treatment prior to BeCl2 intake resulted in significant increase in mRNA expressions of catalase and superoxide dismutase genes near to normalcy. The haematological and biochemical parameters were restorec near to normal levels. Our results suggested that, BeCl2 induced oxidation of cellular lipids and protein; and that administration of crocin reduced BeCl2-induced oxidative stress combined with initiation of mRNA expression of antioxidant genes. (c) 2012 Elsevier Inc. All rights reserved.