PROTEIN SYNTHESIS IN SEA URCHIN EGGS - A LATE RESPONSE TO FERTILIZATION
PROTEIN SYNTHESIS IN SEA URCHIN EGGS - A LATE RESPONSE TO FERTILIZATION
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DOI：
10.1073/pnas.57.4.899
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发表时间：
1967-01-01
影响因子：
11.1
通讯作者：
EPEL, D
中科院分区：
文献类型：
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作者：
EPEL, D
结果.-未受精卵中氨基酸的吸收和掺入：未受精卵在 0.5-7.3 X 10-6 M C'4 标记的亮氨酸或缬氨酸中孵育 5 分钟，按体积计算，其所含氨基酸分别比外部培养基中原始存在的氨基酸多 8.1-1.3 倍。本文未提供的数据表明，转运系统在微摩尔水平下接近饱和，亮氨酸的转运速率高于缬氨酸，并且摄取速率最初是线性的。
Results.-Amino acid uptake and incorporation in unfertilized eggs: Unfertilized eggs, incubated for five minutes in 0.5-7.3 X 10-6 M C'4-labeled leucine or valine, respectively contain 8.1-1.3 times more amino acid, on a volume basis, than was originally present in the external medium. Data not herein presented show that the transport system approaches saturation at micromolar levels, that leucine is transported at a greater rate than is valine, and that the uptake rate is initially linear.