Characterization and comparison of post-natal rat Achilles tendon-derived stem cells at different development stages.
Characterization and comparison of post-natal rat Achilles tendon-derived stem cells at different development stages.
复制标题
大鼠出生后不同发育阶段跟腱来源干细胞的表征和比较
DOI：
10.1038/srep22946
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发表时间：
2016-03-14
影响因子：
4.6
通讯作者：
Ouyang H
中科院分区：
文献类型：
--
作者：
Chen J;Zhang W;Liu Z;Zhu T;Shen W;Ran J;Tang Q;Gong X;Backman LJ;Chen X;Chen X;Wen F;Ouyang H
肌腱干/祖细胞是肌腱组织工程的潜在细胞来源。肌腱组织在发育过程中形态结构的显著变化表明了不同发育阶段TSPC的复杂性。本研究旨在表征和比较出生后大鼠跟腱组织和不同发育阶段的TSPCs。肌腱组织在发育过程中表现出明显的差异：随着时间的推移，组织结构变得致密而规则，细胞核呈梭形，细胞数量减少。与来自1天和56天组织的TSPCs相比，来自7天跟腱组织的TSPCs显示出最高的自我更新能力、细胞增殖和向间充质谱系分化的潜力。微阵列数据显示来自7天跟腱组织的TSPCs中的几组基因上调，这可能是该特定发育阶段独特细胞特征的原因。我们的研究结果表明，与来自1天和56天组织的TSPCs相比，来自7天跟腱组织的TSPCs是上级细胞来源，证明了选择合适的干细胞来源对于有效的肌腱组织工程和再生的重要性。
Tendon stem/progenitor cells (TSPCs) are a potential cell source for tendon tissue engineering. The striking morphological and structural changes of tendon tissue during development indicate the complexity of TSPCs at different stages. This study aims to characterize and compare post-natal rat Achilles tendon tissue and TSPCs at different stages of development. The tendon tissue showed distinct differences during development: the tissue structure became denser and more regular, the nuclei became spindle-shaped and the cell number decreased with time. TSPCs derived from 7 day Achilles tendon tissue showed the highest self-renewal ability, cell proliferation, and differentiation potential towards mesenchymal lineage, compared to TSPCs derived from 1 day and 56 day tissue. Microarray data showed up-regulation of several groups of genes in TSPCs derived from 7 day Achilles tendon tissue, which may account for the unique cell characteristics during this specific stage of development. Our results indicate that TSPCs derived from 7 day Achilles tendon tissue is a superior cell source as compared to TSPCs derived from 1 day and 56 day tissue, demonstrating the importance of choosing a suitable stem cell source for effective tendon tissue engineering and regeneration.